Chonnam Med J.
2001 Jun;37(2):149-157.
Expression of EBNA-1 and EBNA-2 in Epstein-Barr Virus Associated Tumors
- Affiliations
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- 1Department of Pathology, Chonnam University Medical School, Kwangju, Korea.
- 2Department of Oral Pathology, College of Dentistry, Chonnam National University, Kwangju, Korea.
- 3Department of Pathology, College of Medicine, Seonam University, Namwon, Korea.
- 4Department of Parasitology, College of Medicine, Seonam University, Namwon, Korea.
Abstract
- Expression of Epstein-Barr virus (EBV) genome in EBV associated tumors was examined by immuno-histochemistry (IHC) and in situ hybridization (ISH) with biotinylated synthetic oligonucleotide probes specific to EBV-specific nuclear antigen-1 and -2 (EBNA-1 and EBNA-2) mRNA. Conventionally processed and EBV-encoded small RNA (EBER)-positive paraffin sections of 17 Hodgkin's disease (HD), 51 nasopharyngeal carcinoma (NPC) and 2 gastric adenocarcinoma (GC) were used. EBNA-1 protein was detected from 9 cases (53%) of HD, 37 cases (73%) of NPC and none of GC by IHC. Using ISH with probe specific to EBNA-1 mRNA, the EBV was detected in 5 cases (29%) of HD, 20 cases (39%) of NPC and none of GC. No EBNA-2 expression was observed in these tumors. These results indicated that DNA-RNA ISH with oligonucleotide probe could have potentials to detect EBNA-1 mRNA in some EBV-associated tumors, but its sensitivity is lower than conventional IHC.
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