Korean J Obstet Gynecol.  2005 Sep;48(9):2148-2156.

The relationship between interleukin-6 gene G(-634)C polymorphism and change in bone mineral density after hormone replacement therapy in postmenopausal Korean women

Affiliations
  • 1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr

Abstract


OBJECTIVE
To investigate the relationship among the G(-634)C polymorphism in interleukin-6 (IL-6) gene, change in production of IL-6 by whole blood cells (WBCs), and bone mineral density (BMD) after hormone replacement therapy (HRT) in postmenopausal Korean women.
METHODS
The IL-6 G(-634)C polymorphism was analyzed by restriction fragment length polymorphsim (REFLP) in 194 postmenopausal Korean women receiving sequential HRT for 1 year. IL-6 and soluble IL-6 receptor (sIL-6r) produced by WBCs cultured with lipopolysaccharide for 2 days, and serum CrossLaps (CTX) and osteocalcin were measured using enzyme-linked immunosorbent assay (ELISA) and immunoassay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry.
RESULTS
The annual percent changes of BMD were not associated with IL-6 genotypes, and the distribution of IL-6 genotypes were not different between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year). After HRT of 6 months, an increase in IL-6 production by WBCs, without change in sIL-6r production was noted. There were no differences in the 6 month changes of bone turnover markers and IL-6 production by WBCs after HRT across IL-6 genotypes. Change in IL-6 production by WBCs after HRT of 6 month did not correlated with changes of bone turnover markers, but correlated negatively with annual change of BMD at trochanter after HRT.
CONCLUSION
The IL-6 G(-634)C polymorphism did not associate with change in BMD after HRT in postmenopausal Korean women, and did not affect change in the production of IL-6 and sIL-6r by WBCs.

Keyword

Postmenopausal women; IL-6 gene; G(-634)C polymorphism; BMD; HRT

MeSH Terms

Absorptiometry, Photon
Blood Cells
Bone Density*
Enzyme-Linked Immunosorbent Assay
Female
Femur
Genotype
Hormone Replacement Therapy*
Humans
Immunoassay
Interleukin-6*
Osteocalcin
Receptors, Interleukin-6
Spine
Interleukin-6
Osteocalcin
Receptors, Interleukin-6
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