Korean J Obstet Gynecol.  2005 Aug;48(8):1950-1961.

The relationship between interleukin-1 system gene polymorphism and change in bone mineral density after hormone replacement therapy in postmenopausal Korean women

Affiliations
  • 1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr

Abstract


OBJECTIVE
To investigate the relationship between interleukin-1 (IL-1) system gene polymorphisms, and change in production of IL-1 by whole blood cells (WBCs), and bone mineral density (BMD) after hormone replacement therapy (HRT) in postmenopausal Korean women.
METHODS
The IL-1alpha C(-889)T polymorphism, IL-1beta C(-511)T polymorphism and 86-base pair variable number tandem repeat (VNTR) polymorphism in the IL-1 receptor antagonist (IL-1ra) gene was analyzed by restriction fragment length polymorphsim (REFLP), polymerase chain reaction-Genescan, and DNA sequencing in 206 postmenopausal Korean women receiving sequential HRT for 1 year. IL-1alpha, IL-1beta, and IL-1ra produced by WBCs cultured with lipopolysaccharide for 2 days, and serum CrossLaps (CTX), and osteocalcin were measured using enzyme-linked immunosorbent assay (ELISA) and immunoassay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry.
RESULTS
The IL-1 system genotypes were not distributed differently between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year), and no differences in the annual percent change of BMD after HRT were noted among IL-1 system genotypes. After HRT, the production of IL-1alpha by WBCs decreased but no changes in the production of other components of IL-1 system were noted. There were no differences in the 6 month changes of bone turnover markers and the production of IL-1 system components by WBCs and IL-1beta/IL-1ra ratio after HRT across IL-1 system genotypes. Except a negative correlation between IL-1alpha with annual change of BMD at trochanter after HRT, the production of other components of IL-1 system by WBCs and their changes after HRT of 6 month did not correlated with annual change of BMD after HRT
CONCLUSION
The IL-1 system gene polymorphisms do not affect change in BMD, and change in the production of ILs by WBCs after HRT in postmenopausal Korean women.

Keyword

Postmenopausal women; IL-1 system; Gene polymorphism; Bone mineral density; Hormone therapy
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