Korean J Obstet Gynecol.  2004 Apr;47(4):656-662.

The Relationship between Parathyroid Hormone Gene BstBI Polymorphism, Bone Mineral Density and Bone Responsiveness to Hormone Replacement Therapy in Postmenopausal Korean Women

• Kim JG
• Ku SY
• Kim SH
• Choi YM
• Moon SY
• Lee JY

Abstract

OBJECTIVE
To evaluate the relationship between parathyroid hormone (PTH) gene BstBI polymorphism, bone mineral density (BMD) and bone responsiveness to hormone replacement therapy (HRT).
METHODS
PTH BstBI polymorphism was determined by restriction fragment length polymorphism (RFLP) in 444 postmenopausal Korean women. Among these women, 309 women received sequential HRT for 1 year. Serum bone alkaline phosphatase, CrossLaps, osteocalcin, calcitonin, and parathyroid hormone levels were measured by immunoassay and serum calcium and phosphorus levels by atomic absorptiometry. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry before and after HRT of 1 year.
RESULTS
PTH genotype frequencies were 81.1% for BB, 18.0% for Bb, and 1.2% for bb (uppercase letters signifying the absence and lowercase letters the presence of the restriction site). BMD at the femoral neck in women with the bb genotype was higher than that in women with the Bb or BB genotype respectively. Similar trend was found in BMD of lumbar spine and Ward's triangle. The PTH genotypes were not distributed differently between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year) and were not related with annual percent change of BMD after HRT. There were no significant differences in levels of PTH, calcitonin, calcium, phophorus and bone turnover markers or their 6 month percentage changes after HRT among PTH genotypes.
CONCLUSION
PTH BstBI polymorphism is not associated with bone responsiveness to HRT but BMD in Korean women.

Keyword

Postmenopausal women; Parathyroid hormone; BstBI polymorphism; BMD; HRT

MeSH Terms

Absorptiometry, Photon
Alkaline Phosphatase
Bone Density*
Calcitonin
Calcium
Female
Femur
Femur Neck
Genotype
Hormone Replacement Therapy*
Humans
Immunoassay
Osteocalcin
Parathyroid Hormone*
Phosphorus
Polymorphism, Restriction Fragment Length
Spine
Alkaline Phosphatase
Calcitonin
Calcium
Osteocalcin
Parathyroid Hormone
Phosphorus