Korean J Orthod.  1995 Jun;25(3):333-339.

The effect of 1,25-(OH)2D3 on the proliferation and alkaline phosphatase activity of human periodontal ligament cells

  • 1Department of Orthodontics, School of Dentistry Wonkwang University, Korea.
  • 2Department of Dental Therapeutics College of Dentistry Seoul National University, Korea.


The hormonally active vitamin D metabolite, 1,25-dihydroxy vitamin D3 [1.25-(OH)2D3] is one of the several humoral factors that may regulate osteoblast differentiation. The purpose of this study was to evaluate the effects of 1,25-(OH)2D3 on the PDL cells. Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and they were incubated in the environment of 37degrees C, 5% CO2 and 95% humidity. [3H]-thymidine incorporation as a measure of proliferation potential and alkaline phosphatase activity were evaluated at 10nM, l00nM 1,25-(OH)2D3. The observed results were as follows. 1. 1,25-(OH)2D3 was significantly enhanced [3H]-thymidine incorporation at l00nM, But did not affect by 10nM. 2. 1,25-(OH)2D3 was significantly increased alkaline phosphatase activity at 1 day and 6 days in a dose-dependent manner.


Periodontal Ligament; Alkaline Phosphatase; 1,25-(OH)2D3; DNA Synthesis
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