The effects of continuous and intermittent compressive pressure on alkaline phosphatase activity of periodontal ligament cells

Kwon, Suk Yee; Bae, Seong Min; Kyung, Hee Moon; Sung, Jae Hyun

Abstract

The propose of this study was to evaluate the effect of cellular activity of PDL cells dependent on intermittent and continuous compressive force by determining the alkaline phosphatase activity. An intermittent and continuous compressive forces were applied on PDL cells at the confluent stage. The alkaline phosphatase activity was measured on control and experimental groups every 24, 48, 72hours. The experimental group were consist of continous and intermittent compressive group which were compressed by 300g/cm2 of diaphragm pump. The intermittent compressive group was connected by timer which was worked on 10 minutes and off 10minutes. The results were as follow; 1. The alkaline phosphatase activity of intermittent compressive group was lower than control group at 24 hours(P<0.05). 2. The alkaline phosphatase activity between each groups showed no significant difference at 48hours. 3. The alkaline phosphatase activity of continuous compressive group was significantly higher than control group at 72 hours(P<0.01).