Korean J Nutr.  2010 Apr;43(2):105-113. 10.4163/kjn.2010.43.2.105.

The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract

Affiliations
  • 1Jangheungun Mushroom Research Institute, Jeonnam 529-851, Korea.
  • 2Institute of Biomedical Engeneering Research, Wonkwang University, Iksan 570-711, Korea.
  • 3Institute for Better Living, Wonkwang University, Iksan 570-711, Korea.
  • 4Department of Food & Nutrition, Institute for Better Living, Wonkwang University, Iksan 570-711, Korea. ehhwang@wku.ac.kr

Abstract

Ixeris dentata var. albiflora Nakai, a herbal plant, is often used to make a strong stomach as an antiphlogistic used when dyspepsia and to improve appetite in Korea and China. And also it is used for adult diseases such as diabetes and liver diseases as Korean traditional medicine. In this study, the composition and DPPH radical scavenging activities of the root of Ixeris dentata var. albiflora Nakai and its effects on cell viability on vero and chang cells were investigated. Moisture, crude ash, crude protein and crude lipid were 79.14, 2.49, 8.28 and 2.56 g/100 g respectively. The highest mineral content was K. The major free sugars were glucose, fructose and sucrose. Major fatty acid are linoleic acid, palmic acid and linolenic acid. Major amino acids were glutamic acid, arginine and aspartic acid and the total contents of amino acids were 28.12 mg/g. The methanol extracts were further fractionated with n-hexane, chloroform, ethylacetate, butanol and water to get an active fraction. In addition, cell viabilities in each fraction were determined. Methanol extract, butanol, and aqueous fraction showed strong survival rates in vero cell and chang cell viability test, and hexane, chloroform, and ethylacetate fraction were examined for toxin in a cell. The root of Ixeris dentata var. albiflora Nakai had scavenging activities against DPPH radicals in a dose-dependent assay. Ethylacetate fraction's SC50 was 6.8 microgram/mL, very strong DPPH radical scavenging activities, but water fraction did not show any activity.

Keyword

root of Ixeris dentata var. albiflora Nakai; composition; cell viability; DPPH radical scavenging activities

MeSH Terms

Adult
alpha-Linolenic Acid
Amino Acids
Appetite
Arginine
Aspartic Acid
Asteraceae
Carbohydrates
Cell Survival
China
Chloroform
Dyspepsia
Fructose
Glucose
Glutamic Acid
Hexanes
Humans
Korea
Linoleic Acid
Liver Diseases
Medicine, Korean Traditional
Methanol
Plants
Stomach
Sucrose
Survival Rate
Vero Cells
Water
Amino Acids
Arginine
Aspartic Acid
Carbohydrates
Chloroform
Fructose
Glucose
Glutamic Acid
Hexanes
Linoleic Acid
Methanol
Sucrose
Water
alpha-Linolenic Acid
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