Nat Prod Sci.  2023 Dec;29(4):281-286. 10.20307/nps.2023.29.4.281.

Optimization of the Extraction Process for Bioactive Compounds from the Root Barks of Moringa oleifera

• Giang DH
• Hien TV
• Thu Minh NT
• Uyen NT
• Duong HT
• Nhat Le BT
• Thu Ha MT
• Dat NT

• KMID: 2550564
• DOI: http://doi.org/10.20307/nps.2023.29.4.281

Abstract

The optimal condition for Moringa oleifera root barks extraction was determined using response surface methodology and Box-Behnken Design. The actual optimal condition of the factors was 65 o C, ethanol 60%, 40 (mL/g) liquid-to-solid ratio with 240 minutes extraction time. The enrichment of phenolic compounds sharply affected the antioxidant, and inhibitions of α-amylase enzyme, as well as, the anti-inflammatory effect of the extract from M. oleifera root barks. The extract in the optimal condition exhibited better 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and α-amylase inhibitory activities than those of positive controls. Also, the extract showed weak hydroxyl free radical scavenging and nitric oxide (NO) production inhibitory effects. These revealed a simple and promising method for the preparation of bioactive products from the root bark of M. oleifera.

Keyword

Moringa oleifera; Phenolic; Antioxidant; Anti-inflammation; α-Amylase