Korean J Obstet Gynecol.  2002 Mar;45(3):443-449.

Influence of Transforming Growth Factor-alpha on Expression of Matrix metalloproteinase-2 and Matrix metalloproteinase-9 mRNA in Mouse Embryos

• Kim CH
• Kim SH
• Park EJ
• Hong SH
• Chae HD
• Kang BM

Abstract

OBJECTIVE
To investigate the influence of transforming growth factor-alpha (TGF-alpha) on the expression of Matrix metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9) mRNA in mouse embryos. MATERIALS AND METHOD: Eight-cell stage mouse embryos were cultured for 48hours with TGF-alpha at concentrations of 1, 10 and 100 ng/ml. Embryos not treated with TGF-alpha served as control. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to examine the expression of MMP-2 and MMP-9 mRNA in developed blastocysts. Following reverse transcription, strategically designed nested primers, optimized for specificity, were used for amplification from the cDNA equivalent of a single embryo. The products were then verified by restriction enzyme digestion and sequence analysis. Results were analyzed with analysis of variance (ANOVA) and statistical significance was defined as p<0.05.
RESULTS
The relative quantities (relative volume x intensity) of MMP-2 mRNA expressed in embryos of 10 and 100 ng/m of TGF-alpha treatment groups were significantly increased than in the control and 1 ng/ml of TGF-alpha treatment group (67.2+/-7.5 and 77.4+/-11.6 vs. 38.6+/-4.5 and 43.4+/-6.1, p<0.001). The relative quantities of MMP-9 mRNA of 100 ng/ml TGF-alpha treatment group was significantly increased than in the control and 1 ng/ml TGF-alpha treatment groups (67.6+/-6.5 vs. 36.6+/-14.2 and 40.2+/-11.3, p<0.001, p<0.01, respectively).
CONCLUSION
This study suggests that TGF-alpha itself may induce the expression of MMP-2 and 9 mRNA in mouse embryos.

Keyword

Transforming growth factor-alpha (TGF-alpha); Matrix metalloproteinase-2 (MMP-2); Matrix metalloproteinase-9 (MMP-9); Reverse transcription-polymerase chain reaction (RT-PCR)

MeSH Terms

Animals
Blastocyst
Digestion
DNA, Complementary
Embryonic Structures*
Matrix Metalloproteinase 2*
Matrix Metalloproteinase 9*
Mice*
Reverse Transcription
RNA, Messenger*
Sensitivity and Specificity
Sequence Analysis
Transforming Growth Factor alpha
DNA, Complementary
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
RNA, Messenger
Transforming Growth Factor alpha