Korean J Nephrol.  2008 May;27(3):290-298.

Apoptosis of Podocyte and Role of TGF-beta1 LAP in Unilateral Ureteral Obstruction in Mice

• Lee WD
• Kang MS
• Kang SW
• Kim YW
• Kim YH

Abstract

PURPOSE
It was well known that transforming growth factor (TGF)-beta1 plays a pivotal role in interstitial fibrosis and loss of podocyte. We explored the effects of exogenous administration of TGF-beta1 latency-associated peptide (LAP) in a model of renal fibrosis induced by unilateral ureteral obstruction (UUO) and examined whether TGF-beta1 LAP can inhibit apoptosis of podocyte.
METHODS
Twenty four male BALB/c mice were unilaterally obstructed of proximal ureters by ligation. Half of the mice with operation and half of 8 control were administered recombinant human LAP intraperitoneally. One to three mice per group were euthanized on days 3, 7, 14, and 21 after operation for observation of renal fibrosis and apoptosis of podocyte.
RESULTS
Interstitial fibrosis was less severe in LAP-treated group. Obstructed kidneys from LAP- untreated mice had more glomerular apoptotic podocytes (TUNEL assay) compared to LAP-treated mice at day 7, 14, and 21 after operation.
CONCLUSION
Intraperitoneal administration of TGF-beta1 LAP prevents the loss of podocyte & renal damage partially up to day 14 after operation.

Keyword

Apoptosis; Podocyte; Transforming growth factor beta 1; Ureteral obstructi

MeSH Terms

Animals
Apoptosis
Fibrosis
Humans
Kidney
Ligation
Male
Mice
Podocytes
Transforming Growth Factor beta1
Transforming Growth Factors
Ureter
Ureteral Obstruction
Transforming Growth Factor beta1
Transforming Growth Factors