Korean J Nephrol.  2011 Sep;30(5):443-451.

The Role of Adipose Tissue-Derived Stem Cells in Ischemic Acute Renal Injury in Rats

Affiliations
  • 1Department of Pathology, Catholic University of Daegu, School of Medicine, Daegu, Korea.
  • 2Department of Pathology, Yeungnam University, College of Medicine, Daegu, Korea. yyjjkim@yun.ac.kr
  • 3Department of Pediatrics, Yeungnam University, College of Medicine, Daegu, Korea.
  • 4Department of Clinical Pathology, Tae Kyeung College, Gyeongsan, Korea.

Abstract

PURPOSE
The aim of this study is to explore the role of human adipose tissue-derived adult stem cells (ASCs) in repair of acute ischemic renal injury.
METHODS
Acute ischemic tubular necrosis was induced in Spraque-Dawley rats (n=15) by clamping bilateral renal pedicles for 40 minutes. Before releasing the clamps, iron labeled stem cells (1x106/0.3 mL) were directly injected to one side of renal cortex and phosphate-buffered saline (PBS) injections were done as a control to the opposite kidney. The examination on both kidney was done after 1, 2 and 4 weeks (n=5 each). The evaluation of the degree of tubular necrosis and the location of stem cells was done by light and electron microscopically.
RESULTS
The rats were all alive without rejection. Morphologically, recovery rate was increased up to about 20% in ASCs injection group in 2 weeks-group (p<0.001). Few Prussian-blue positive cells were demonstrated in 2 weeks-group and more cells were present in 4 weeks-group. The cells which were positively stained in Prussian-blue stain were mainly located in peritubular spaces. Electron microscopically, fine iron granules with electron density were found in the cytoplasm of peritubular endothelial cells.
CONCLUSION
As a result, the endothelial cells might be differentiated from injected ASCs. It seems that stem cells help repairing the tubular epithelium in acute renal failure model, not by tubulogenesis but by vasculogenesis. This outcome suggests that the possibility of therapeutic use of ASCs without immunosuppression of recipients in acute ischemic renal injury.

Keyword

Mesenchymal stem cells; Acute renal failure

MeSH Terms

Acute Kidney Injury
Adult Stem Cells
Animals
Constriction
Cytoplasm
Electrons
Endothelial Cells
Epithelium
Humans
Immunosuppression
Iron
Kidney
Light
Mesenchymal Stromal Cells
Necrosis
Rats
Rejection (Psychology)
Stem Cells
Iron
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