Korean J Clin Pathol.  1999 Dec;19(6):642-646.

Analysis of Apolipoprotein E Genotypes in Several Demential diseases

• Park YM
• Jeon YM
• Kwon GC
• Koo SH
• Park JW

Abstract

BACKGROUND
An increased frequency of the epsilon4 allele in Alzheimer's disease has been reported, which suggested a functional role of apolipoprotein E isoforms in pathophysiology of Alzheimer's disease. The aims of this study were to determine the frequency of ApoE genotypes in various types of dementia such as Alzheimer's disease, vascular dementia, and Parkinson's disease, to relate epsilon4 and assess risk factors for different types of dementia.
METHODS
We assessed the frequency of Apolipoprotein E alleles in 29 patients with Alzheimer's disease, 10 patients with vascular dementia and 9 demented patients with Parkinson's disease. The Apolipoprotein E genotype was determined the polymerase chain reaction (PCR) with sequence-specific oligonuculeotide primers (SSOP) and reverse hybridiza- tion using the INNO-LiPA (Line Probe Assay) Apo E typing kit.
RESULTS
Allele frequencies of epsilon2, epsilon3, and epsilon4 were : 0.5, 0.72, and 0.23 in Alzheimer's disease; 0, 0.65, and 0.35 in vascular dementia; 0.6, 0.83 and 0.11 in demented patients with Parkinson's disease. Conculsion : These results indicate that the apolipoprotein E4 allele is associated with not only Alzheimer's disease but also vascular dementia, however not associated with Parkinson's disease. No such an association was observed between the apolipoprotein E alleles and age.

Keyword

Alzheimer's disease; vascular dementia; Parkinson's diseases. Apolipoprotein E genotypes

MeSH Terms

Alleles
Alzheimer Disease
Apolipoprotein E4
Apolipoproteins E
Apolipoproteins*
Dementia
Dementia, Vascular
Gene Frequency
Genotype*
Humans
Parkinson Disease
Polymerase Chain Reaction
Protein Isoforms
Risk Factors
Apolipoprotein E4
Apolipoproteins
Apolipoproteins E
Protein Isoforms