Korean J Pediatr.  2008 Jan;51(1):42-46. 10.3345/kjp.2008.51.1.42.

Association of apolipoprotein E polymorphisms with serum lipid profiles in obese adolescent

• Yoon JM
• Lim JW
• Cheon EJ
• Ko KO

• KMID: 1487157
• DOI: http://doi.org/10.3345/kjp.2008.51.1.42

Abstract

PURPOSE: Apolipoprotein E (Apo E) plays a major role in lipoprotein metabolism and lipid transport. Many investigators have described that Apo E polymorphisms is one of the most important genetic determinants for cardiovascular disease. The purpose of this study was to evaluate the association between Apo E polymorphisms and serum lipid profiles in obese adolescent.
METHODS
We measured the serum concentrations of glucose, apolipoprotein (Apo) A1, Apo B, total cholesterol (TC), triglyceride (TG), HDL and LDL-cholesterol after overnight fasting in obese adolescent. Apo E polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTS
86 obese adolescents participated in this study. The body mass index (BMI) of participants were excess of 95 percentile by age and sex. Male to female ratio was 1.7 and mean age of study group was 16.2+/-1.8 years. Mean BMI was 27.4+/-2.5 kg/m2. The frequency of epsilon2, epsilon3 and epsilon4 allele were 8.1%, 87.2% and 4.7% respectively. Study populations were classified into the following three genotypes 1) Apo E2 group (n=13, 15.1%) carrying either the epsilon2/epsilon2 or epsilon2/epsilon3 2) Apo E3 group (n=65, 75.6%) carrying the most frequent epsilon3/epsilon3 3) Apo E4 group (n=8, 9.3%) carrying either the epsilon3/epsilon4 or epsilon4/epsilon4. No differences were found among Apo E genotypes concerning age, sex, weight, height and BMI. Apo B and LDL-cholesterol concentrations were significantly higher in the Apo E4 group (P<0.05). No association were found between Apo E genotypes and glucose, Apo A1, TC, TG and HDL.
CONCLUSIONS
We confirmed that serum concentrations Apo B and LDL-cholesterol were influenced by Apo E genotypes. Apo E polymorphisms seems to influence some alteration of lipid metabolism associated with obesity in adolescent.

Keyword

Apolipoprotein E; Polymorphism; Obesity

MeSH Terms

Adolescent
Alleles
Apolipoprotein A-I
Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Apolipoproteins
Apolipoproteins B
Apolipoproteins E
Body Mass Index
Cardiovascular Diseases
Cholesterol
Fasting
Female
Genotype
Glucose
Humans
Lifting
Lipid Metabolism
Lipoproteins
Male
Obesity
Research Personnel
Apolipoprotein A-I
Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Apolipoproteins
Apolipoproteins B
Apolipoproteins E
Cholesterol
Glucose
Lipoproteins