Korean Circ J.  2004 Dec;34(12):1158-1166. 10.4070/kcj.2004.34.12.1158.

The Impact of Apolipoprotein A-I Polymorphisms on the Lipid Profiles in Middle Aged Healthy Men and Women

• Moon JY
• Cho EY
• Kim WH
• Choi SH
• Ko YG
• Park HY
• Lee JH
• Lee JE
• Jang YS

• KMID: 2227164
• DOI: http://doi.org/10.4070/kcj.2004.34.12.1158

Abstract

BACKGROUND AND OBJECTIVES
Apolipoprotein A-I is the major lipoprotein constituent of high density lipoprotein in plasma. In this study, the role of two polymorphisms in the apo A-I gene was investigated on the serum lipid profiles and apo A-I levels in healthy men and women.
SUBJECTS AND METHODS
Blood samples were obtained from 417 subjects (M : F=169 : 248, mean age 47.2 years). The apo A-I genotypes were determined by SNP-IT assays using the SNPstream 25KTM system.
RESULTS
The frequencies of the A allele at the XmnI restriction site and position -75 bp were 0.25/0.23 and 0.19/0.17 in men and women, respectively. A strong positive linkage disequilibrium (D'=0.990) between two polymorphisms was detected. In men, the A allele at the XmnI restriction site was associated with significantly lower levels of triglyceride (p=0.028) compared to the G/G subjects, but no significant associations were detected between the G-75A polymorphism and any of the lipid traits examined. In women, each A allele for the XmnI restriction site and -75 bp polymorphisms were significantly associated with higher levels of apo A-I (p=0.032 and p=0.012). In the multiple regression analysis, the HDL, being a current drinker and the A allele of the XmnI restriction site polymorphism were major determinants of the serum apo A-I levels in women (R2=0.272, p<0.05).
CONCLUSION
Our study showed that the A allele at XmnI restriction site in the apo A-I gene was associated with decreased triglyceride levels in men. Each A allele of two polymorphisms was associated with an elevated apo A-I level in women.

Keyword

Apolipoprotein A-I; Polymorphism; Triglyceride; HDL-cholesterol

MeSH Terms

Alleles
Apolipoprotein A-I*
Apolipoproteins*
Female
Genotype
Humans
Linkage Disequilibrium
Lipoproteins
Male
Middle Aged*
Plasma
Triglycerides
Apolipoprotein A-I
Apolipoproteins
Lipoproteins