Korean J Anat.  2007 Sep;40(3):227-233.

Effect of Propofol on Kupffer Cell Superoxide Dismutase Activities and Cytoprotections during Hypoxia-Reoxygenation

• Noh YC
• Song IH
• Kim JY
• Seong CR
• Jee DL
• Sung EG

Abstract

The aims of this study were to verify the hypoxia-reoxygenation injury of primary cultured Kupffer cells and the effect of propofol against the hypoxia-reoxygenation injury through quantitating lactate dehydrogenase (LDH) release and superoxide dismutase (SOD) activity.The sequential treatments with hypoxia and reoxygenation induced significant increasement of LDH release (P.0.01) and decresement of SOD activity(P.0.05) in primary cultured Kupffer cell. The level of LDH release and SOD activity after sequential treatments with hypoxia and reoxygenation were restored to the control level by the propofol treatment in the concentration of 0.5 and 5 microgram/mL. Propofol in concentration of 50 microgram/mL induced significant increasement of LDH release (P.0.01) on both normal culture and hypoxia-reoxygenation culture of the Kupffer cell. As hypoxia and reoxygenation procedures and propofol treatment were concurrently added to the cultured Kupffer cell, propofol treatment in the concentration of 50 microgram/mL decreased significantly the SOD activity (P.0.01). In conclusion, propofol in this hypoxia-reoxygenation model could provide a valuable clue for the study of liver transplantation and of propofol.

Keyword

Kupffer cell; Hypoxia-Reoxygenation; Propofol; Lactate dehydrogenase (LDH); Superoxide dismutase (SOD) activity

MeSH Terms

Anoxia
Kupffer Cells
L-Lactate Dehydrogenase
Liver Transplantation
Propofol*
Superoxide Dismutase*
Superoxides*
L-Lactate Dehydrogenase
Propofol
Superoxide Dismutase
Superoxides