J Rhinol.  1999 May;6(1):24-29.

Expression of GABA Transporters in the Rat's Olfactory Bulb during Development

• Park YJ
• Lee MY
• Yoon HR

Abstract

Developmental changes in the expression of two GABA (gamma-aminobutyric acid) transporter proteins, GAT-1 and GAT-3, in the olfactory bulb of embryonic and postnatal rats were examined with immunocytochemistry using antisera against GAT-1 and GAT-3. The expression and localization of GAT-1 and GAT-3 showed distinct temporal patterns during olfactory bulb development. GAT-1 immunoreactivity appeared weakly in most likely growing axons of the presumptive glomerular layer from embryonic day 18 and increased during the first postnatal week. In contrast, GAT-3 immunoreactivity, first detected at E16, was found in radial glial cell fascicles and was replaced by what were likely astroglial cells postnatally. At P7, GAT-1 and GAT-3 immunoreactivities reached the adult pattern i.e., GAT-1 immunoreactivity was observed in the labeled punctate structures in all layers of the olfactory bulb except the nerve fiber layer, while GAT-3 immunoreactivity was observed in the astroglial processes of all layers of the olfactory bulb. Our results suggest that GABA transporters, especially GAT-3, play important roles in regulating the GABA levels of developing olfactory bulbs.

Keyword

GABA transporter (GAT-1, GAT-3); Immunocytochemistry; Olfactory bulb; Development; Rat

MeSH Terms

Adult
Animals
Axons
Ependymoglial Cells
GABA Plasma Membrane Transport Proteins*
gamma-Aminobutyric Acid*
Humans
Immune Sera
Immunohistochemistry
Nerve Fibers
Olfactory Bulb*
Rats
GABA Plasma Membrane Transport Proteins
Immune Sera
gamma-Aminobutyric Acid