Korean J Anat.  1999 Dec;32(6):837-847.

Effects of kainic Acid-induced seizures on GABA and GABA transporter in the cerebellum of the rat

Affiliations
  • 1Department of Anatomy, Ulsan University College of Medicine, Seoul, Korea.

Abstract

The effects of kainic acid-induced seizures on GABA and GABA transporter in the rat cerebellum were examined by means of immunohistochemical and Western blot methods. Immunocytochemical analysis showed that kainic acid-induced seizures led to a decreased immunoreactivity for GABA to 3 weeks after seizures with an slight increase in the immunoreactivity of cerebellum 24 h after treatment. Immunoreactivities of GABA transporters, GAT-1 and GAT-3 which are localized neurons and astrocytes, were increased at 24 and 48h and after that weak immunoreactivites for GABA transporters were shown in the cerebellar tissues. Our results indicate that kainic acid-induced seizures exerts specific effects on GABA contents and the GABA transporters in the cerebellum and a decrease of GABA contents might not always associated with the decrease in the number of GABA transporters in the rat cerebellum.

Keyword

Kainic acid; Seizures; GABA; GABA transporter; GAT-1; GAT-3

MeSH Terms

Animals
Astrocytes
Blotting, Western
Cerebellum*
GABA Plasma Membrane Transport Proteins
gamma-Aminobutyric Acid*
Kainic Acid
Neurons
Rats*
Seizures*
GABA Plasma Membrane Transport Proteins
Kainic Acid
gamma-Aminobutyric Acid
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