Yeungnam Univ J Med.  1994 Dec;11(2):314-322. 10.12701/yujm.1994.11.2.314.

Effect of GABA on the Contractility of Canine Trachealis Muscle

• Koo CH
• Kwon OC
• Choi EM
• Lee KY
• Kim WJ

• KMID: 2352247
• DOI: http://doi.org/10.12701/yujm.1994.11.2.314

Abstract

This study aimed to investigate the existence of GABA receptor and the mechanisms of action of GABA and diazepam of the trachealis muscle isolated from dog. Horizontal muscle strips of 2mm×15mm were prepared from canine trachea, and isometric myography in isolated muscle chamber bubbled with 95/5%-O₂/CO₂ at 36℃, at the pH of 7.4 was performed. Muscle strips contracted responding to the electrical field stimulation (ESP) by 2~20 Hz, 20 msec, monophasic square wave of 60 VDC. GABA and diazepam suppressed the EFS-induced contractions to the similar extent, significantly. (p<0.05). Bicuculline, a GABA(A) receptor antagonist blocked both GABA- and diazepam-inhibitions; but DAVA, a GABA(B) receptor antagoinst did not affect either of them. These results suggest than in the canine trachealis muscle, there may be only GABA(A) receptor, and GABA and diazepam inhibit the contractility via GABA(A) receptor.

MeSH Terms

Animals
Bicuculline
Diazepam
Dogs
gamma-Aminobutyric Acid*
Hydrogen-Ion Concentration
Myography
Receptors, GABA
Receptors, GABA-A
Trachea
Bicuculline
Diazepam
Receptors, GABA
Receptors, GABA-A
gamma-Aminobutyric Acid