J Korean Ophthalmol Soc.  2005 Feb;46(2):257-270.

Inhibitory Effects of Imatinib Mesylate on Transdifferentiation of Lens Epithelial Cells into Fibroblast

Affiliations
  • 1Department of Ophthalmology, Inha University, College of Medicine, Inchon, Korea. jhoh9707@hanmail.net
  • 2Dr. Tea's Eye Clinic, Anyang, Korea.
  • 3Incheon Medical Center, Inchon, Korea.

Abstract

PURPOSE
To investigate whether Imatinib mesylate (IM) could inhibit the transdifferentiation of the lens epithelial cells (LECs) into fibroblasts using the capsular bag model. METHODS: In the capsular bag model, LECs were cultured by exposure to IM at various concentrations for 3 min. The effect of IM was analysed by observing the covering area, numbers of alpha-SMA positive cell, and BrdU incorporated proliferating cells. The same analysis was performed in the culture of LECs with TGF-beta. RESULTS: The covering area was significantly decreased by the treatment of 30 micro M IM, and the positive cells for alpha-SMA and BrdU were also decreased by IM treatment in a dose dependent manner. In addition, increasing TGF-beta concentration accelerated transdifferentiation, but suppressed the acceleration of the transdifferentiation induced by TGF-beta. CONCLUSIONS: In the capsular bag model, IM effectively inhibited not only the transdifferentiation of LECs into fibroblasts but also the TGF-beta induced acceleration of the transdifferentiation.

Keyword

alpha-Smooth muscle actin; Capsular bag model; Fibroblast; Imatinib mesylate; Transdifferentiation

MeSH Terms

Acceleration
Bromodeoxyuridine
Epithelial Cells*
Fibroblasts*
Mesylates*
Transforming Growth Factor beta
Imatinib Mesylate
Bromodeoxyuridine
Mesylates
Transforming Growth Factor beta
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