J Korean Pediatr Soc.  2000 Jun;43(6):746-754.

Protective Effect of Probucol against Adriamycin-Induced Apoptosis in Cultured Rat Cardiac Myocytes

• Lee SJ
• Lee DS
• Jung YW

Abstract

PURPOSE: In adriamycin(ADR)-induced cardiomyopathy, several different mechanisms are suggested. However, little information is available regarding the role of apoptosis. In the present study, we examined the induction of apoptosis on ADR treatment and anti-apoptotic effects of probucol, a lipid-lowering drug, and we also studied the changes of bcl-2 expression in order to see the molecular mechanisms underlying the effect of probucol.
METHODS
Cardiac myocytes were isolated from 3-day-old rats, and cultured in low(1 pM) or high doses(10pM) of ADR for 24 hours. Probucol(50 pM) was added 30 minutes before ADR administration. Apoptosis was determined by TUNEL staining, and bcl-2 expression was estimated by immunocytochemistry.
RESULTS
The number of TUNEL-positive cells significantly increased in both groups treated with ADR. However, anti-apoptotic effect of probucol was evident only in low dose. In addition, the expression of bcl-2 was significantly increased only in the low-dose ADR treatment group and its expression was inhibited by pretreatment of probucol. CONCLUSION: These results suggest that apoptosis might play an important role in ADR-induced cardiotoxicity, and ADR-induced apoptosis was partially prevented by pretreatment of probucol. And ADR-induced apoptosis was not related with depression of bcl-2. Additionally, inhibition of bcl-2 gene expression of low-dose ADR treatment group by probucol suggests that another cell survival mechanism could be implicated in the action of probucol. (J Korean Pediatr Soc 2000;43:746-754)

Keyword

Adriamycin; Apoptosis; Probucol; Bcl-2; Cardiac myocytes

MeSH Terms

Animals
Apoptosis*
Cardiomyopathies
Cell Survival
Depression
Doxorubicin
Genes, bcl-2
Immunohistochemistry
In Situ Nick-End Labeling
Myocytes, Cardiac*
Probucol*
Rats*
Doxorubicin
Probucol