J Korean Surg Soc.
1997 Jun;52(6):839-845.
Detection of p53 Gene Mutations in Gastric cancers: Polymerase Chain Reaction and Single Strand Conformational Polymorphism Migration Technique
- Affiliations
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- 1Department of Surgery, Chonnam National University Medical College, Korea.
- 2Research Institute of Clinical Medicine, Chonnam National University Medical College, Korea.
- 3Department of Clinical Pathology, Chonnam National University, Korea.
- 4Department of Surgery, Seoul University Medical College, Korea.
Abstract
- Abberations of the p53 gene in 24 primary gastric carcinomas were examined by single-strand conformation polymorphism analysis of polymerase chain reaction products and by immunohistochemical staining with monoclonal antibody. Of these gastric adenocarcinomas, 23 were advanced gastric carcinomas, and 1 was early gastric cancer. Using polymerase chain reaction (PCR) and a single-strand conformation polymorphism migration technique (SSCP), the presence of mutations in exons 4-9 was evaluated. Using the mouse specific anti-human p53 monoclonal antibody, we also looked for overexpression of the p53 protein in tissue sections. In 5 cases shifted bands were reproducibly identified by PCR-SSCP, and all mutations were in exon 4 and 5,6. Thirteen of the tumor samples were positively stained with the monoclonal antibody. In only one of the 5 mutated cases a positive immunostaining was observed, and we couldn't find the any correlation between the mutations detected by PCR-SSCP and immunostaining. The presence of a p53 mutation by PCR-SSCP was associated with lower PCNA labeling index ( mean = 40.8:75.8%). In the cases with overexpression of p53 protein, the pathologic stages were more advanced than without overexpression of p53 protein (p=0.037). The mutation of p53 was not correlated with mutations of other oncogenes such as c-myc and c-Ha ras. Our results in this group of patients suggest that p53 mutations detected by PCR-SSCP should be reevaluated, and p53 mutations detected by immunostaining was more reliable.
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