J Menopausal Med.  2015 Aug;21(2):89-92. 10.6118/jmm.2015.21.2.89.

Expression of Vitamin D Receptor in Seminal Vesicles of Cholesterol Formula Mice

Affiliations
  • 1Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea. heeobgy@schmc.ac.kr
  • 2Department of Urology, Soonchunhyang University College of Medicine, Bucheon, Korea.
  • 3Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Cheonan, Korea.
  • 4Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang University, Asan, Korea.

Abstract


OBJECTIVES
Genomic function of vitamin D receptor (VDR) indicates spermatogenesis that is important for in male reproductive organ authors evaluated the VDR expression in seminal vesicles with high cholesterol (HC) formula diet rat, because there is no report about relationship or difference in VDR in seminal vesicles between HC and control.
METHODS
Male C57BL/6 mice aged 5 weeks were raised for 13 weeks. After one week of adaptation-period, they were fed different diet on normal AIN-93G diet, or HC diet containing 2% cholesterol for 12 weeks. The antibodies used were rabbit anti-VDR primary polyclonal.
RESULTS
There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.
CONCLUSION
Our data give the no difference in expression of VDR of seminal vesicles rat between HC formula diet and normal AIN-93G diet. But we confirmed the VDR expression in seminal vesicles.

Keyword

Cholesterol; Seminal vesicles; Vitamin D receptor

MeSH Terms

Animals
Antibodies
Body Weight
Cholesterol*
Diet
Humans
Male
Mice*
Rats
Receptors, Calcitriol*
Seminal Vesicles*
Spermatogenesis
Vitamin D*
Vitamins*
Antibodies
Cholesterol
Receptors, Calcitriol
Vitamin D
Vitamins

Figure

  • Fig. 1 Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.


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Jae Hyung Ahn, Yoo Hun Noh, Kyung Joo Um, Hyo Sun Kim, Sook Cho
J Menopausal Med. 2018;24(2):119-126.    doi: 10.6118/jmm.2018.24.2.119.

Influence of Supplementary Vitamin D on Bone Mineral Density When Used in Combination with Selective Estrogen Receptor Modulators
Hyun Ju Liu, Soo Ah Kim, Da Joung Shim, Ji Min Jung, Eun Jeong Lee
J Menopausal Med. 2019;25(2):94-99.    doi: 10.6118/jmm.19193.


Reference

1. Aquila S, Guido C, Perrotta I, Tripepi S, Nastro A, Ando S. Human sperm anatomy: ultrastructural localization of 1alpha,25-dihydroxyvitamin D receptor and its possible role in the human male gamete. J Anat. 2008; 213:555–564.
2. Bouillon R, Carmeliet G, Verlinden L, van Etten E, Verstuyf A, Luderer HF, et al. Vitamin D and human health: lessons from vitamin D receptor null mice. Endocr Rev. 2008; 29:726–776.
3. Blomberg Jensen M, Nielsen JE, Jorgensen A, Rajpert-De Meyts E, Kristensen DM, Jorgensen N, et al. Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract. Hum Reprod. 2010; 25:1303–1311.
4. Cross NL. Role of cholesterol in sperm capacitation. Biol Reprod. 1998; 59:7–11.
5. Lee HH. A role of vitamin D in postmenopausal women. J Korean Soc Menopause. 2008; 14:109–114.
6. Kim TH, Park J, Lee HH, Lee WS, Chung SH, Park Y, et al. Expression of vitamin D receptor by pulse consumption in the uterus of menopausal mouse model. J Korean Soc Menopause. 2013; 19:1–8.
7. Rho HK, Lee ES, Park HM. Perception level of vitamin D and calcium on osteoporosis treatment. J Korean Soc Menopause. 2008; 14:115–129.
8. Huggins C, Scott WW, Heinen JH. Chemical composition of human semen and of the secretions of the prostate and seminal vesicles. Am J Physiol. 1942; 136:467–473.
9. Groen JJ, Balogh M, Yaron E, Freeman J. Influence of the nature of the fat in diets high in carbohydrate (mainly derived from bread) on the serum cholesterol. Am J Clin Nutr. 1965; 17:296–304.
10. Muthusami KR, Chinnaswamy P. Effect of chronic alcoholism on male fertility hormones and semen quality. Fertil Steril. 2005; 84:919–924.
11. Oh J, Weng S, Felton SK, Bhandare S, Riek A, Butler B, et al. 1,25(OH)2 vitamin d inhibits foam cell formation and suppresses macrophage cholesterol uptake in patients with type 2 diabetes mellitus. Circulation. 2009; 120:687–698.
12. Makishima M. Nuclear receptors as targets for drug development: regulation of cholesterol and bile acid metabolism by nuclear receptors. J Pharmacol Sci. 2005; 97:177–183.
13. Wang JH, Keisala T, Solakivi T, Minasyan A, Kalueff AV, Tuohimaa P. Serum cholesterol and expression of ApoAI, LXRbeta and SREBP2 in vitamin D receptor knock-out mice. J Steroid Biochem Mol Biol. 2009; 113:222–226.
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