J Korean Soc Pediatr Endocrinol.  2000 Jun;5(1):45-51.

Expression of Growth Hormone Receptor Gene During 3T3-L1 Differentiation

• Yu JH

Abstract

PURPOSE: Growth hormone(GH) produces a variety of effects in adipose tissue via GHRs on the cell membrane. In mouse, alternative splicing of the nascent transcript from the GHR gene produces two major transcripts:GHR mRNA and GHR binding protein(GHBP) mRNA. These two transcripts share the common extracellular ligand-binding domain, but differ in the C-terminal sequence. Since GHR plays an important role in mediating the actions of GH in adipose metabolism, I initiated these studies to examine GHR gene expression during mouse 3T3-Ll preadipocyte-adipocyte differentiation.
METHODS
GHR and GHBP transcripts were detected by RNase protection assay (RPA) using the antisense riboprobes complementary either to the specific sequence of the GHR or to the sequence shared by both GHR and GHBP mRNAs. RNA prepared from 3T3-L1 cells at day 0(preadipocytes) and day 7(adipocytes) after treatment with actinomycin D was analyzed by RPA.
RESULTS
After stimulation of differentiation, mRNA abundance increased 25-fold and reached a maximal level by day 7 of adipogenesis. The GHR mRNA:GHBP mRNA ratio was 1.3+/-.15 and remained unchanged during differentiation. The decay rate for both mRNAs, estimated by treating the cells with actinomycin D, was approximately 24 hours and showed no significant difference between preadipocytes and adipocytes.
CONCLUSION
GHR gene expression is upregulated during preadipocyte-adipocyte differentiation.

Keyword

GHR; Gene expression; Preadipocyte-adipocyte differentiation

MeSH Terms

3T3-L1 Cells
Adipocytes
Adipogenesis
Adipose Tissue
Alternative Splicing
Animals
Cell Membrane
Dactinomycin
Gene Expression
Growth Hormone*
Metabolism
Mice
Negotiating
Receptors, Somatotropin*
Ribonucleases
RNA
RNA, Messenger
Dactinomycin
Growth Hormone
RNA
RNA, Messenger
Receptors, Somatotropin
Ribonucleases