J Korean Assoc Maxillofac Plast Reconstr Surg.
2010 Nov;32(6):504-510.
Proliferation and Functional Activity of Human Adipose Tissue-Derived CD146 Positive Endothelial Cells According to Culture Mediums
- Affiliations
-
- 1Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21), Jinju, Korea. surbyun@gsnu.ac.kr
- 2Clinical Research Institute, Gyeongsang National University Hospital, Jinju, Korea.
- 3Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21), Jinju, Korea.
- 4Department of Oral and Maxillofacial Surgery, School of Dentistry, Busan National University, Yangsan, Korea.
- 5Maxillofacial Center, Onhospital, Busan, Korea.
Abstract
- PURPOSE
This study was to examine the proliferation and function of the adipose tissue-derived endothelial cells according to different culture medium conditions.
MATERIALS AND METHODS
Adipose tissue-derived CD146 positive endothelial cells were cultured in according to different culture mediums (DMEM culture medium with or without osteogenic inductive agents and EBM-2 culture medium with or without osteogenic inductive agents). The proliferation and function of the adipose tissue-derived endothelial cells was examined in different culture medium conditions.
RESULTS
Adipose tissue-derived endothelial cells formed tube-like structures on Matrigel in EBM-2 culture medium with or without osteogenic inductive agents. However, the cells did not form tube-like structures on Matrigel in DMEM medium with or without osteogenic inductive agents. After 24 hours of culture, among the culture medium using EBM-2, the proliferation of the cells were promoted in EBM-2 medium without osteogenic inductive agents than in EBM-2 medium with osteogenic inductive agents. However, 72 hours of culture, the proliferation of the cells were promoted in EBM-2 medium with osteogenic inductive agents than in EBM-2 medium without osteogenic inductive agents.
CONCLUSION
These results suggest that the proliferation and function of the adipose tissue-derived CD146 positive endothelial cells could be maintained in EBM-2 with osteogenic inductive agents.
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