J Korean Acad Conserv Dent.  2010 Jan;35(1):5-12. 10.5395/JKACD.2010.35.1.005.

Pulp response of beagle dog to direct pulp capping materials: Histological study

Affiliations
  • 1Department of Conservative Dentistry, Seoul National University Bundang Hospital, Korea. yhchoi@snubh.org
  • 2Department of Oral Maxillofacial Surgery, Seoul National University Bundang Hospital, Korea.
  • 3Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea.

Abstract

The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs'pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: ProRoot(R) MTA, Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed. In MTA, calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration. In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

Keyword

Direct pulp capping; Pulp inflammation; Dentinal bridge; Histological evaluation; Beagle dog

MeSH Terms

Adhesives
Aluminum Compounds
Animals
Calcium
Calcium Compounds
Calcium Hydroxide
Composite Resins
Dental Pulp Capping
Dentin
Dogs
Drug Combinations
Glutamates
Guanine
Hydroxides
Jordan
Minerals
Necrosis
Oxides
Polymethyl Methacrylate
Resin Cements
Silicates
Tooth
Pemetrexed
Adhesives
Aluminum Compounds
Calcium
Calcium Compounds
Calcium Hydroxide
Composite Resins
Drug Combinations
Glutamates
Guanine
Hydroxides
Minerals
Oxides
Polymethyl Methacrylate
Resin Cements
Silicates
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