J Korean Breast Cancer Soc.
1998 Dec;1(2):192-202. 10.4048/jkbcs.1998.1.2.192.
The Effect of Tamoxifen of the Estrogen Receptor cDNA-Iipofected MDA-MB-231 Human Breast Cancer Cells
- Affiliations
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- 1Department of Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- KMID: 2173677
- DOI: http://doi.org/10.4048/jkbcs.1998.1.2.192
Abstract
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BACKGROUND: The loss of estrogen and progesterone receptors appears to be associated with a progression to less-differentiated and hormone-independent tumors. The gain of hormone independency over time even in estrogen receptor-positive tumors has become another obstacle to endocrine therapy for breast cancer. We tried to regain the hormone dependency in estrogen receptor-negative breast cancer cells by lipofecting estrogen receptor cDNA.
MATERIALS AND METHODS
The mutant human estrogen receptor cDNA (pSG5-HE0) was lipofected into estrogen receptor-negative human breast cancer cell line MDA-MB-231, in an attempt to restore their sensitivity to antiestrogen. Then the effects if 17 beta -estradiol and tamoxifen were studied by counting viable cell numbers after treating the lipofected cell line with either one or together.
RESULTS
The cell growth was most profoundly inhibited in 4 days after lipofection with mutant human estrogen receptor cDNA, which was overcome after that days. Tamoxifen, as an antiestrogen, showed a growth inhibitory effect slightly strong over combined conditions of tamoxifen and 17 beta estradiol compared to estrogen-treated group and to control.
CONCLUSIONS
The temporary induction of estrogen receptor by lipofection with pSG5-HE0 on estrogen receptor-negative human breast cancer cell line MDA-MB-231 showed negative growth control on these cells by tamoxifen, indicating that liposome-mediated estrogen receptor transfection may be used as a novel therapeutic strategy for hormone independent human breast cancers in the near future.
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