Biomol Ther.  2016 Mar;24(2):123-131. 10.4062/biomolther.2015.106.

Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling

• Pengjam Y
• Madhyastha H
• Madhyastha R
• Yamaguchi
• Nakajima Y
• Maruyama M

• KMID: 2170523
• DOI: http://doi.org/10.4062/biomolther.2015.106

Abstract

Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside, aloin, on osteogenic induction of MC3T3-E1 cells. Aloin increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts. Aloin also increased the ALP activity in adult human adipose-derived stem cells (hADSC), indicating that the action of aloin was not cell-type specific. Alizarin red S staining revealed a significant amount of calcium deposition in cells treated with aloin. Aloin enhanced the expression of osteoblast differentiation genes, Bmp-2, Runx2 and collagen 1a, in a dose-dependent manner. Western blot analysis revealed that noggin and inhibitors of p38 MAPK and SAPK/JNK signals attenuated aloin-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt-5a signaling pathway also annulled the influence of aloin, indicating Wnt-5a dependent activity. Inhibition of the different signal pathways abrogated the influence of aloin on ALP activity, confirming that aloin induced MC3T3-E1 cells into osteoblasts through MAPK mediated Wnt and Bmp signaling pathway.

Keyword

Aloin; MC3T3-E1 cell; MAPK; Wnt; Bmp signaling

MeSH Terms

Adult
Alkaline Phosphatase
Blotting, Western
Calcium
Collagen
Core Binding Factor Alpha 1 Subunit
Humans
Osteoblasts
Osteoporosis
p38 Mitogen-Activated Protein Kinases
Pathology
Quality of Life
RNA, Small Interfering
Signal Transduction
Stem Cells
Alkaline Phosphatase
Calcium
Collagen
Core Binding Factor Alpha 1 Subunit
RNA, Small Interfering
p38 Mitogen-Activated Protein Kinases