Immune Netw.  2006 Mar;6(1):13-19. 10.4110/in.2006.6.1.13.

Granulocyte Colony Stimulating Factor (G-CSF) Attenuates 2,4,6-Trinitrobenzene Sulfonic Acid (TNBS)-induced Colitis in Mice

• Choi EY
• Jun CD
• Oh JM
• Kim YR
• Lee ST
• Kim SW

• KMID: 2167984
• DOI: http://doi.org/10.4110/in.2006.6.1.13

Abstract

BACKGROUND: Granulocyte colony stimulating factor (G-CSF) is known as a cytokine central to the hematopoiesis of blood cells and to modulate their cellular functions. Besides granulocytes and their precursors, monocytes/macrophages and endothelial cells are direct target cells of G-CSF action. G-CSF influences immune cells in an anti-inflammatory way.
METHODS
To evaluate whether G-CSF has a potential for preventing or ameliorating diseases characterized by mucosal inflammation, we used a mouse model with trinitrobenzene sulfonic acid (TNBS)-induced inflammatory colitis. To the mice model G-CSF was administrated daily by intraperitoneal injection. Macroscopic evaluation and immunohistochemical analysis of colonic tissues were performed.
RESULTS
Recombinant human G-CSF significantly inhibited LPS-induced TNF-alpha mRNA expression in THP-1 cells. As for in vivo relevance, G-CSF dramatically reduced the weight loss of mice, colonic damage, and mucosal ulceration that characterize TNBS colitis. Moreover, G-CSF suppressed the expression of tumor necrosis factor-alpha, interleukin-1beta, and intercellular adhesion molecule-1 in TNBS colitis.
CONCLUSION
Current results demonstrate that G-CSF may be an effective agent for the treatment of diseases characterized by mucosal inflammation.

Keyword

G-CSF; inflammatory bowel diseases; inflammation; TNBS

MeSH Terms

Animals
Blood Cells
Colitis*
Colon
Colony-Stimulating Factors*
Endothelial Cells
Granulocyte Colony-Stimulating Factor
Granulocytes*
Hematopoiesis
Humans
Inflammation
Inflammatory Bowel Diseases
Injections, Intraperitoneal
Intercellular Adhesion Molecule-1
Interleukin-1beta
Mice*
RNA, Messenger
Tumor Necrosis Factor-alpha
Ulcer
Weight Loss
Colony-Stimulating Factors
Granulocyte Colony-Stimulating Factor
Intercellular Adhesion Molecule-1
Interleukin-1beta
RNA, Messenger
Tumor Necrosis Factor-alpha