Allergy Asthma Immunol Res.  2013 Jan;5(1):34-41. 10.4168/aair.2013.5.1.34.

Association Analysis Between FILIP1 Polymorphisms and Aspirin Hypersensitivity in Korean Asthmatics

• Kim JY
• Kim JH
• Park BL
• Pasaje CF
• Bae JS
• Park JS
• Jang AS
• Uh ST
• Kim YH
• Kim MK
• Choi IS
• Cho SH
• Choi BW
• Park CS
• Shin HD

• KMID: 2167004
• DOI: http://doi.org/10.4168/aair.2013.5.1.34

Abstract

PURPOSE
Aspirin exacerbated respiratory disease (AERD) results in a severe asthma attack after aspirin ingestion in asthmatics. The filamin A interacting protein 1 (FILIP1) may play a crucial role in AERD pathogenesis by mediating T cell activation and membrane rearrangement. We investigated the association of FILIP1 variations with AERD and the fall rate of forced expiratory volume in one second (FEV1).
METHODS
A total of 34 common FILIP1 single nucleotide polymorphisms (SNPs) were genotyped in 592 Korean asthmatic subjects that included 163 AERD patients and 429 aspirin-tolerant asthma (ATA) controls.
RESULTS
This study found that 5 SNPs (P=0.006-0.01) and 2 haplotypes (P=0.01-0.03) of FILIP1 showed nominal signals; however, corrections for the multiple testing revealed no significant associations with the development of AERD (P corr>0.05). In addition, association analysis of the genetic variants with the fall rate of FEV1, an important diagnostic marker of AERD, revealed no significant evidence (P corr>0.05).
CONCLUSIONS
Although further replications and functional evaluations are needed, our preliminary findings suggest that genetic variants of FILIP1 might be not associated with the onset of AERD.

Keyword

Filamin A interacting protein 1; single nucleotide polymorphism; haplotype; asthma; aspirin exacerbated respiratory disease

MeSH Terms

Aspirin
Asthma
Contractile Proteins
Eating
Forced Expiratory Volume
Haplotypes
Humans
Hypersensitivity
Membranes
Microfilament Proteins
Negotiating
Polymorphism, Single Nucleotide
Aspirin
Contractile Proteins
Microfilament Proteins

Figure

• 

  Fig. 1 Gene maps and haplotypes of the FILIP1. (A) A map of FILIP1 on chromosome 6q14.1, in which coding exons, UTR, and SNPs are shown. Coding exon (Ex) is marked by shaded blocks while UTRs are marked by white blocks. (B) Haplotypes of FILIP1 in the Korean population. SNPs are divided into 2 haplotype blocks. Only common haplotypes (frequency>0.05) were used for an association analysis.

• 

  Fig. 2 LD coefficients graphical plot (|D'| and r2) among SNPs based on the genotypes of Korean study subjects (n=592).

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