Genomics Inform.  2006 Mar;4(1):11-15.

Identification of hRad21-Binding Sites in Human Chromosome

• Chin C
• Chung BS

Abstract

The aim of this study is to identify hRad21-binding sites in human chromosome, the core component of cohesin complex that held sister chromatids together. After chromatin immunoprecipitation with an hRad21 antibody, it was cloned the recovered DNA and sequenced 30 independent clones. Among them, 20 clones (67%) contained repetitive elements including short interspersed transposable elements (SINE or Alu elements), long terminal repeat (LTR) and long interspersed transposable elements (LINE), fourteen of these twenty (70%) repeats clones had Alu elements, which could be categorized as the old and the young Alu Subfamily, eleven of the fourteen (73%) Alu elements belonged to the old Alu Subfamily, and only three Alu elements were categorized as young Alu subfamily. There is no CpG island within these selected clones. Association of hRad21 with Alu was confirmed by chromatin immunoprecipitation-PCR using conserved Alu primers. The primers were designed in the flanking region of Alu, and the specific Alu element was shown in the selected clone. From these experiments, it was demonstrated that hRad21 could bind to SINE, LTRs, and LINE as well as Alu.

Keyword

hRad21 cohesin repeated element; Alu; SINE; LINE; LTR; ChIP; sequence

MeSH Terms

Alu Elements
Chromatids
Chromatin
Chromatin Immunoprecipitation
Chromosomes, Human*
Clone Cells
CpG Islands
DNA
DNA Transposable Elements
Humans
Humans*
Siblings
Terminal Repeat Sequences
Chromatin
DNA
DNA Transposable Elements