Genomics Inform.  2004 Jun;2(2):67-74.

Changes of Gene Expression in NIH3T3 Cells Exposed to Osmotic and Oxidative Stresses

• Lee JS
• Jung JH
• Kim TH
• Seo JS

Abstract

Cells consistently face stressful conditions, which cause them to modulate a variety of intracellular processes and adapt to these environmental changes via regulation of gene expression. Hyperosmotic and oxidative stresses are significant stressors that induce cellular damage, and finally cell death. In this study, oligonucleotide microarrays were employed to investigate mRNA level changes in cells exposed to hyperosmotic or oxidative conditions. In addition, since heat shock protein 70 (HSP70) is one of the most inducible stress proteins and plays pivotal role to protect cells against stressful condition, we performed microarray analysis in HSP70 overexpressing cells to identify the genes expressed in a HSP70 dependent manner. Under hyperosmotic or oxidative stress conditions, a variety of genes showed altered expression. Down regulation of protein phosphatase1 beta (PP1 beta) and sphingosine 1 phosphate phosphatase 1 (SPPase1) was detected in both stress conditions. Microarray analysis of HSP70 overexpressing cells demonstrated that diverse mRNA species depend on the level of cellular HSP70. Genes encoding lysyl oxidase, thrombospondin 1, and procollagen displayed altered expression in all tested conditions. The results of this study will be useful to construct networks of stress response genes.

Keyword

microarray; hyperosmotic stress; oxidative stress; HSP70

MeSH Terms

Cell Death
Down-Regulation
Gene Expression Regulation
Gene Expression*
Heat-Shock Proteins
HSP70 Heat-Shock Proteins
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Oxidative Stress*
Procollagen
Protein-Lysine 6-Oxidase
RNA, Messenger
Sphingosine
Thrombospondin 1
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Procollagen
Protein-Lysine 6-Oxidase
RNA, Messenger
Sphingosine
Thrombospondin 1