Int J Stem Cells.  2016 May;9(1):124-136. 10.15283/ijsc.2016.9.1.124.

Characterization of Senescence of Culture-expanded Human Adipose-derived Mesenchymal Stem Cells

Affiliations
  • 1Latvian Biomedical Research and Study Centre, Riga, Latvia. dianal@biomed.lu.lv
  • 2Children's Clinical University Hospital, Clinical Laboratory, Riga, Latvia.

Abstract

BACKGROUND AND OBJECTIVES
Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates in regenerative medicine. The need for in vitro propagation to obtain therapeutic quantities of the cells imposes a risk of impaired functionality due to cellular senescence. The aim of the study was to analyze in vitro senescence of previously cryopreserved human ADSCs subjected to serial passages in cell culture.
METHODS AND RESULTS
ADSC cultures from 8 donors were cultivated until proliferation arrest was reached. A gradual decline of ADSC fitness was observed by altered cell morphology, loss of proliferative, clonogenic and differentiation abilities and increased β-galactosidase expression all of which occurred in a donor-specific manner. Relative telomere length (RTL) analysis revealed that only three tested cultures encountered replicative senescence. The presence of two ADSC subsets with significantly different RTL and cell size was discovered. The heterogeneity of ADSC cultures was supported by the intermittent nature of aging seen in tested samples.
CONCLUSION
We conclude that the onset of in vitro senescence of ADSCs is a strongly donor-specific process which is complicated by the intricate dynamics of cell subsets present in ADSC population. This complexity needs to be carefully considered when elaborating protocols for personalized cellular therapy.

Keyword

Human adipose-derived mesenchymal stem cells; Serial passage; Cell aging; Relative telomere length; Subpopulations; Heterogeneity

MeSH Terms

Aging*
Cell Aging
Cell Culture Techniques
Cell Size
Humans*
Mesenchymal Stromal Cells*
Population Characteristics
Regenerative Medicine
Serial Passage
Telomere
Tissue Donors
Full Text Links
  • IJSC
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr