J Korean Hip Soc.  2008 Sep;20(3):209-214. 10.5371/jkhs.2008.20.3.209.

Activation of Transcription Factor NF-kappaB and c-Jun/AP-1 by Various Compositions of Particles in Osteoclast Precursor Cells

• Kim YS
• Kwon SY
• Han SK
• Kwon JY
• Choi NY

• KMID: 2162629
• DOI: http://doi.org/10.5371/jkhs.2008.20.3.209

Abstract

PURPOSE
This study was performed in order to investigate the effects of various particle preparations on NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells.
MATERIALS AND METHODS
Osteoclast precursor cells isolated from C57BL mice were treated with PMMA (polymethylmethacrylate) spheres, polystyrene, titanium particles, and retrieved metal particles from failed cementless total hip replacements. NF-kappaB and c-Jun/AP-1 DNA binding activities were analyzed using electrophoretic mobility shift assays (EMSA).
RESULTS
Commercially available PMMA and polystyrene spheres routinely showed negativity on endotoxin assays, but titanium particles and retrieved metal particles consistently showed positivity. PMMA spheres, with a maximal response noted at 30 minutes with an optimal concentration of 0.6 mg/ml, were potent stimulator of NF-kappaB and c-Jun/AP-1 activity in osteoclast precursor cells. Other particles (polystyrene, titanium, metal retrievals) also activated transcription factor NF-kappaB and c-Jun/AP-1 compared to controls. Endotoxin removal from retrieved metal particles diminished the biologic effect by approximately 40%.
CONCLUSION
Particles of various compositions and sizes (PMMA, polystyrene, titanium, and retrieved metal particles) activated the NF-kappaB and c-Jun/AP-1 signaling pathways. This suggests that NF-kappaB and c-Jun/AP-1 may have important roles in the pathogenesis of periprosthetic osteolysis.

Keyword

Transcription factor; NF-kappaB; c-Jun/AP-1; Particle; Osteoclast precursor cell; Electrophoretic mobility shift assay

MeSH Terms

Animals
Arthroplasty, Replacement, Hip
DNA
Electrophoretic Mobility Shift Assay
Mice
Mice, Inbred C57BL
NF-kappa B
Osteoclasts
Osteolysis
Polymethyl Methacrylate
Polystyrenes
Titanium
Transcription Factors
DNA
NF-kappa B
Polymethyl Methacrylate
Polystyrenes
Titanium
Transcription Factors