Exp Mol Med.  2014 Jan;46(1):e71. 10.1038/emm.2013.136.

Exogenous rhTRX reduces lipid accumulation under LPS-induced inflammation

• Han GY
• Lee EK
• Park HW
• Kim HJ
• Kim CW

• KMID: 2155336
• DOI: http://doi.org/10.1038/emm.2013.136

Abstract

Redox-regulating molecule, recombinant human thioredoxin (rhTRX) which shows anti-inflammatory, and anti-oxidative effects against lipopolysaccharide (LPS)-stimulated inflammation and regulate protein expression levels. LPS-induced reactive oxygen intermediates (ROI) and NO production were inhibited by exogenous rhTRX. We identified up/downregulated intracellular proteins under the LPS-treated condition in exogenous rhTRX-treated A375 cells compared with non-LPS-treated cells via 2-DE proteomic analysis. Also, we quantitatively measured cytokines of in vivo mouse inflammation models using cytometry bead array. Exogenous rhTRX inhibited LPS-stimulated production of ROI and NO levels. TIP47 and ATP synthase may influence the inflammation-related lipid accumulation by affecting lipid metabolism. The modulation of skin redox environments during inflammation is most likely to prevent alterations in lipid metabolism through upregulation of TIP47 and ATP synthase and downregulation of inflammatory cytokines. Our results demonstrate that exogenous rhTRX has anti-inflammatory properties and intracellular regulatory activity in vivo and in vitro. Monitoring of LPS-stimulated pro-inflammatory conditions treated with rhTRX in A375 cells could be useful for diagnosis and follow-up of inflammation reduction related with candidate proteins. These results have a therapeutic role in skin inflammation therapy.

Keyword

inflammation; lipid accumulation; rhTRX; skin cell proteomics; TIP47

MeSH Terms

Animals
Antioxidants/*pharmacology
Cell Line, Tumor
Humans
Inflammation/metabolism
*Lipid Metabolism
Lipopolysaccharides/pharmacology
Mice
Mice, Inbred C57BL
Nitric Oxide/metabolism
Proteome/genetics/metabolism
Skin/drug effects/metabolism/pathology
Thioredoxins/*pharmacology
Antioxidants
Lipopolysaccharides
Proteome
Nitric Oxide
Thioredoxins