Exp Mol Med.  2013 Jul;45(7):e31.

A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis

• Park SM
• Park SJ
• Kim HJ
• Kwon OH
• Kang TW
• Sohn HA
• Kim SK
• Moo Noh S
• Song KS
• Jang SJ
• Sung Kim Y
• Kim SY

Abstract

Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.

Keyword

gastric cancer; high-throughput RNA sequencing; long intergenic non-coding RNA; non-coding RNA; RNA-seq

MeSH Terms

Animals
DNA, Intergenic/genetics
Expressed Sequence Tags/*metabolism
Extracellular Space/metabolism
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genetic Predisposition to Disease
Humans
Male
Matrix Metalloproteinase 9/metabolism
Mice
Mice, Inbred C57BL
Multivariate Analysis
Neoplasm Metastasis
Neoplasm Staging
Phenotype
Proportional Hazards Models
RNA, Long Noncoding/*genetics/metabolism
RNA, Messenger/genetics/metabolism
Reproducibility of Results
Stomach Neoplasms/*genetics/*pathology
Survival Analysis
DNA, Intergenic
RNA, Long Noncoding
RNA, Messenger
Matrix Metalloproteinase 9