J Korean Soc Coloproctol.  2007 Apr;23(2):116-128. 10.3393/jksc.2007.23.2.116.

Differential Expressions of Genes in Colorectal Cancer with Liver Metastasis by using a cDNA Microarray Chip: A Pilot Study

Affiliations
  • 1Department of Surgery, Kumi CHA Hospital, College of Medicine, Pochon CHA University, Gumi, Korea.
  • 2Department of Surgery, Kyungpook National University School of Medicine, Daegu, Korea. kyuschoi@mail.knu.ac.kr
  • 3Department of Immunology, Kyungpook National University School of Medicine, Daegu, Korea.
  • 4Biomedical Research Institute, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Korea.

Abstract

PURPOSE: Liver metastasis is the most common type of failure in the treatment of colorectal cancer. The identification of differential expressions of genes in colorectal cancer and liver metastasis is important to differentiate the genetic mechanism of carcinogenesis and liver metastasis from that of a normal mucosa. The aim of this study is to find candidate genes playing roles in liver metastasis of colorectal cancer by using cDNA microarray.
METHODS
We screened a group of genes differentially expressed in a normal mucosa and in cancer and liver metastasis by using a 4.7 K cDNA microarray chip in 8 patients with far advanced colorectal cancer from Jan 2003 to May 2004 at Kyungpook National University Hospital.
RESULTS
A comparison of mRNA expressions of genes in normal mucosa vs. cancer, normal mucosa vs. liver metastasis, and cancer vs. liver metastasis, 76 and 27 known and unknown genes were significantly over-expressed in cancer and liver metastasis, respectively. Also 62 and 26 genes were down- regulated in cancer and liver metastasis. Among those genes, TIMP-1, SRY-box9, Rattus norvegicus fibronectin 1, mitotic check point regulator, etc. were constantly up- regulated in cancer or metastasis, and hsgk, etc. were down-regulated in cancer or liver metastasis. CONSLUSIONS: The cDNA microarray chip technique could be a useful for robust screening of candidate genes involved in carcinogenesis or metastasis of colorectal cancer.

Keyword

Colorectal cancer; Liver metastasis; Gene expression; cDNA microarray chip

MeSH Terms

Animals
Carcinogenesis
Colorectal Neoplasms*
DNA, Complementary*
Fibronectins
Gene Expression
Gyeongsangbuk-do
Humans
Liver*
Mass Screening
Mucous Membrane
Neoplasm Metastasis*
Oligonucleotide Array Sequence Analysis*
Pilot Projects*
Rats
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
DNA, Complementary
Fibronectins
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-1
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