J Korean Dent Sci.  2015 Dec;8(2):74-81. 10.5856/JKDS.2015.8.2.74.

Odontogenic Ameloblast-Associated Protein (Odam) Plays Crucial Roles in Osteoclast Differentiation via Control of Actin Ring Formation

• Lee HK
• Park JC

• KMID: 2154081
• DOI: http://doi.org/10.5856/JKDS.2015.8.2.74

Abstract

PURPOSE
In osteoclast differentiation, actin-rich membrane protrusions play a crucial role in cell adhesion. Odontogenic ameloblast-associated protein (Odam) contributes to cell adhesion by inducing actin rearrangement. Odam-mediated RhoA activity may play a significant role in multinucleation of osteoclasts. However, the precise function of Odam in osteoclast cell adhesion and differentiation remains largely unknown. Here, we identify a critical role for Odam in inducing osteoclast adhesion and differentiation.
MATERIALS AND METHODS
The expression of Odam in osteoclasts was evaluated by immunohistochemistry. Primary mouse bone marrow and RAW264.7 cells were used to test the cell adhesion and actin ring formation induced by Odam. RESULT: Odam was expressed in osteoclasts around alveolar bone. Odam transfection induced actin filament rearrangement and cell adhesion compared with the control or collagen groups. Overexpression of Odam promoted actin stress fiber remodeling and cell adhesion, resulting in increased osteoclast fusion.
CONCLUSION
These results suggest that Odam expression in primary mouse osteoclasts and RAW264.7 cells promotes their adhesion, resulting in the induction of osteoclast differentiation.

Keyword

Cell adhesion; Odam; Osteoclasts; RhoA

MeSH Terms

Actin Cytoskeleton
Actins*
Animals
Bone Marrow
Cell Adhesion
Collagen
Immunohistochemistry
Membranes
Mice
Osteoclasts*
Stress Fibers
Transfection
Actins
Collagen