Immune Netw.  2006 Jun;6(2):67-75. 10.4110/in.2006.6.2.67.

Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc

• Cho HI
• Chwae YJ
• Park SM
• Kim J

• KMID: 2150616
• DOI: http://doi.org/10.4110/in.2006.6.2.67

Abstract

BACKGROUND: Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail.
METHODS
By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells.
RESULTS
The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T cell lysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells.
CONCLUSION
Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.

Keyword

KIR; Shc; SHP-1; SHP-2; cell activation; cell proliferation; inhibitory signal transduction

MeSH Terms

Cell Proliferation
Cytoplasm
Killer Cells, Natural
Phosphotyrosine
Protein Tyrosine Phosphatases
T-Lymphocytes
Transfection
Phosphotyrosine
Protein Tyrosine Phosphatases