Immune Netw.  2001 Apr;1(1):70-76. 10.4110/in.2001.1.1.70.

Usage of T Cell receptor repertoire is restricted in synovial lymphocytes in rheumatoid arthritis

• Kwon DH
• Lee SK
• Kim SJ
• Choi IH

• KMID: 2150559
• DOI: http://doi.org/10.4110/in.2001.1.1.70

Abstract

BACKGROUND: Rheumatoid arthritis is an autoimmune disease characterized by a chronic inflammatory process, primarily involving the synovial membrane of peripheral joints, where T cell activation is found. To address the superantigen stimulation in rheumatoid arthritis, T cell clonality and the expression of activation markers were analyzed.
METHODS
To detect TCRB V usage, inverse PCR and sequencing were done. Monoclonal antibodies were used for flow cytometric analysis of TCRBV8 or TCRBV5. As result s, a restricted usage of TCRBV3 gene was detected in synovial lymphocytes from one rheumatoid arthritis patient . However, preferential usage for TCRB V8, which may be one indicator for stimulation by staphylococcal superantigen, was not obvious although general activation of T cells was found as high DR+ percentage in synovial T cells. These data show specific antigen rather than superantigen might involve the pathogenesis of rheumatoid arthritis.

Keyword

rheumatoid arthritis; synovial membrane; TCR beta - Chain

MeSH Terms

Antibodies, Monoclonal
Arthritis, Rheumatoid*
Autoimmune Diseases
Humans
Joints
Lymphocytes*
Polymerase Chain Reaction
Receptors, Antigen, T-Cell*
Synovial Membrane
T-Lymphocytes
Antibodies, Monoclonal
Receptors, Antigen, T-Cell