J Korean Soc Microbiol.  1999 Jun;34(3):221-231.

Characterization of Streptococcus pyogenes by T Protein Serotype , rRNA Gene Restriction Pattern , and Antimicrobial Susceptibility

• Lee YH
• Ju YR
• Hwang KJ
• Lee KJ
• Park KS
• Bae SM
• Eoum YK
• Cha SH
• Lee CS

Abstract

Fifty strains of Streptococcus pyogenes were isolated from patients with pharyngitis and invasive streptococcal infections in Seoul Korea from February to October, 1998. All isolates were characterized by T protein serotype, rRNA gene restriction pattern and antimicrobial susceptibility. T protein serotype showed 9 kinds in distrubution including T12 and R4, Among the total isolates, 23 strains (46%) belinged to the serotype T12, and 13 strains (26%) to T4. In the resistance rate to six antimicrobial agents including penicillin, the strains showed the highest resistance to crythromycin with 46%, followed by tetracycline with 44%, and clindamycin with 40%, but no strains showed the resistance to penicillin G, vancomycin and ceftriaxone. Of the tewnty three isolates shown resistance to erythromycin 20 strains (87%), had high minimum inhibitory concentration (MIC) of >128 ug/ml, and showed cross-resistance to both clindamycin and tetracycline. And also to clinidamycin, 20 strains had high MIC of > 128 ug/ml. With 17 isolates of serotype T12, it was revealed that three was a high correlation between antimicrobial resistance frequency and serotype In the rRNA gene restriction pattern, the ribotype was classified into fourteen subtypes renged from A to N, the most dominant type E accounted for 24 strains (48%) and the second dominant type D for 12 strains (24%). Of 24 strains of T12 serotype, 19 strains (83%) were E ribotype, whereas of 13 strains of T4 serotype, 12 were D ribotype. Eighty-five percent of the strains shown resistance to 3 antibiotics of erythromycin, clindamycin and letracycline belonged to subtype E. These results indicated that S. pyogenes had reciprocal correlations among T protein serotype, rRNA gene restriction pattern, and antimicrobial susceptibility.

MeSH Terms

Anti-Bacterial Agents
Anti-Infective Agents
Ceftriaxone
Clindamycin
Erythromycin
Genes, rRNA*
Humans
Korea
Microbial Sensitivity Tests
Penicillin G
Penicillins
Pharyngitis
Ribotyping
Seoul
Streptococcal Infections
Streptococcus pyogenes*
Streptococcus*
Tetracycline
Vancomycin
Anti-Bacterial Agents
Anti-Infective Agents
Ceftriaxone
Clindamycin
Erythromycin
Penicillin G
Penicillins
Tetracycline
Vancomycin