Korean J Obstet Gynecol.  2005 Mar;48(3):701-708.

The expression of epidermal growth factor and fibroblast growth factor-2 in the eutopic endometrium of women with endometriosis

• Choi JW
• Lee HA
• Lee SR
• Hong SH
• Nah HY
• Lee YJ
• Kim SH
• Chae HD
• Kim CH
• Kang BM

Abstract

OBJECTIVE
To compare the expression of epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) in the eutopic endometrium of women with and without endometriosis.
METHODS
Thirty-two women with histologically confirmed endometriosis after laparotomy or laparoscopy were recruited. Twenty-eight women with carcinoma in situ of the uterine cervix, uterine myoma and ovarian tumor but no evidence of endometriosis served as control. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified by real time PCR to quantify the expression of EGF, FGF-2, and GAPDH genes.
RESULTS
Including all menstrual phases, the levels of mRNA expressions of EGF and FGF-2 were not different between the eutopic endometrium of women with and without endometriosis. When analyzed separately according to the 6 menstrual phases, no differences were found between the two groups, either.
CONCLUSION
This study shows that the levels of mRNA expressions of EGF and FGF-2 in the eutopic endometrium were not different between women with and without endometriosis.

Keyword

EGF; FGF-2; Endometriosis; Endometrium; Angiogenesis

MeSH Terms

Carcinoma in Situ
Cervix Uteri
DNA, Complementary
Endometriosis*
Endometrium*
Epidermal Growth Factor*
Female
Fibroblast Growth Factor 2*
Fibroblasts*
Humans
Laparoscopy
Laparotomy
Leiomyoma
Real-Time Polymerase Chain Reaction
Reverse Transcription
RNA
RNA, Messenger
DNA, Complementary
Epidermal Growth Factor
Fibroblast Growth Factor 2
RNA
RNA, Messenger