J Korean Ophthalmol Soc.  2003 Jul;44(7):1629-1638.

A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea

• Lee JS
• Lee JR
• Jung SC
• Oum BS

Abstract

PURPOSE
To differentiate the genes that are up-regulated or down-regulated and the cellular morphology in the corneal stromal cells between normal and diabetic rat. METHODS: After isolation with trizol (GIBCO, NY, USA), labeling and purification of complementary DNA(cDNA) probes were carried out using Atlas(TM) Glass Microarrays 1.0. RESULTS: Autoradiographic analysis showed that 35 genes were up-regulated and 8 genes were down-regulated by Atlas Image(TM) Software. Up-regulated genes were as calcium/calmodulin dependent protein kinase I, proteasome 26S subunit 1, prostaglandin F receptor. Down-regulated genes were aquaporin 8, cytochrome c oxidase polypeptide Vb, glypican 1. CONCLUSIONS: There were many differential genes in hormone receptor, voltage gated ion channels, oncogenes and tumor suppressors, G protein between normal and diabetic keratocyte of rat.

Keyword

Atlas(TM) Glass Microarrays 1.0; Diabetic keratocyte; Hybridization

MeSH Terms

Animals
Cornea*
Electron Transport Complex IV
Glass
Glypicans
GTP-Binding Proteins
Ion Channels
Oncogenes
Proteasome Endopeptidase Complex
Protein Kinases
Rats*
Stromal Cells
Electron Transport Complex IV
GTP-Binding Proteins
Glypicans
Ion Channels
Proteasome Endopeptidase Complex
Protein Kinases