J Korean Ophthalmol Soc.  2006 May;47(5):818-827.

Gene Microarray Related with Apoptosis in Diabetic OLETF Keratocytes

• Lee JE
• Kim HS
• Lee JS

Abstract

PURPOSE: This study was to identified differentiated genes concerned with apoptosis that are up-regulated or down-regulated in OLETF corneal keratocytes stimulated with interleukin-1alpha.
METHODS
Otsuka Long Evans Tokushima Fatty (OLETF) corneal keratocytes were primarily cultured and treated with 20 ng/ml of IL-1a for 6 hours. Hybridization was carried out using Oligonucleotide microarray. A sample of normal keratocytes was labeled with Cy 3, and a diabetic keratocytes sample was labeled with Cy 5.
RESULTS
Diabetes showed 20 down-regulated genes and 24 up-regulated genes compared with normal keratocytes. Also, IL-1alpha-treated diabetic keratocytes expressed 31 down-regulated and 33 up-regulated genes. Compared with diabetic keratocytes, the newly expressed genes in OLETF treated with IL-1alpha were Bcl, Lam, Timp, Mmp, Socs, Bmp, Ccl, Lcn, C7, etc.
CONCLUSIONS
There were some genes related with apoptosis that expressed differently between normal and diabetic keratocytes of OLETF. Also, IL-1alpha may stimulate differing effects of apoptosis on diabetic corneal keratocytes. Studies analyzing the apoptotic significance of these differences identified in diabetic keratocytes are needed.

Keyword

Apoptosis; Diabetes mellitus; Keratocyte; Microarray; Rat

MeSH Terms

Animals
Apoptosis*
Corneal Keratocytes
Diabetes Mellitus
Interleukin-1alpha
Oligonucleotide Array Sequence Analysis
Rats
Interleukin-1alpha