Tuberc Respir Dis.  2007 Feb;62(2):105-112. 10.4046/trd.2007.62.2.105.

The Efficacy of alpha-lipoic Acid on the Endotoxin-induced Acute Lung Injury

• Huh JW
• Hong SB
• Kim MJ
• Lim CM
• Koh Y

• KMID: 2114596
• DOI: http://doi.org/10.4046/trd.2007.62.2.105

Abstract

BACKGROUND: Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of alpha-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI.
MATERIALS AND METHODS
ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+alpha-lipoic acid, LPS, and LPS+alpha-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-alpha, IL-1beta, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration.
RESULTS
The total cell and neutrophil counts of the LPS+alpha-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+alpha-lipoic acid groups. The TNF-alpha, IL-1beta, and IL-6 concentrations in the BAL fluid were not decreased by the alpha-lipoic acid treatment in the LPS treated rats.
CONCLUSIONS
Although alpha-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study.

Keyword

alpha-lipoic acid; oxidative stress; acute lung injury; antioxidant

MeSH Terms

Acute Lung Injury*
Animals
Bronchoalveolar Lavage
Interleukin-6
Lung
Models, Animal
Neutrophil Infiltration
Neutrophils
Oxidative Stress
Peroxidase
Rats
Rats, Sprague-Dawley
Thioctic Acid*
Trachea
Tumor Necrosis Factor-alpha
Interleukin-6
Peroxidase
Thioctic Acid
Tumor Necrosis Factor-alpha

Figure

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  Figure 1 The effect of α-lipoic acid on the level of CINC of BAL fluid in rats treated with LPS injection (L: LPS group, LA: LPS+α-lipoic acid group) (*p<0.05)

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  Figure 2 The effect of α-lipoic acid on the level of MIP of BAL fluid in rats treated with LPS injection (L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)

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  Figure 3 The effect of α-lipoic acid on the level of cytokines of BAL fluid in rats treated with LPS (C: control, A: α-lipoic, L: LPS, LA: LPS+α-lipoic acid) (*p<0.05)

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