J Korean Ophthalmol Soc.  1991 Jul;32(7):585-593.

Effect of Bovine Aqueous Humor on Prodution of IL-2 and IL-6, and Other Parameters of Immunoeompetency in Mice

• Cha D
• Park C
• Ha T

Abstract

The injection of antigen into the anterior chamber of the eye results in a unusual pattern of systemic immune responses termed anterior chamber associated immune deviation(ACAID). This study was done to investigate the in vitro effect of bovine aqueous humor(BAR) on the proliferation response of murine spleen and thymus to mitogens, to determine in vivo effects of BAR on delayed-type hypersensitivity and hemagglutinin response to sheep red blood cells, and to examine contact hypersensitivity to dinitrofluorobenzene(DNFB). Also, the effect on in vivo exposure to BAH on the production of IL-2 and IL-6 of murine lymphocyte was investigated. As a result, various concentration of BAR inhibited or enhanced the proliferation response of mouse splenocytes to mitogens. BAR injection significantly enhanced 'Arthus reaction to SRBC, but failed to change DTR reaction to SRBC. Interestingly, however, BAR profoundly inhibited contact hypersensitivity to DNFB and HA response. BAR inhibited both IL-2 and IL-6 production of murine spleen and thymus cells. Taken together, these results suggest that BAR contains soluble factor(s) that can suppress or enhance immune response, depending on experimental conditions and that immunomodulatory activity of BAR is not species-specific.

Keyword

Bovine aqueous humor; Interleukin-2 and 6

MeSH Terms

Animals
Anterior Chamber
Aqueous Humor*
Dermatitis, Contact
Dinitrofluorobenzene
Erythrocytes
Hemagglutinins
Hypersensitivity
Interleukin-2*
Interleukin-6*
Lymphocytes
Mice*
Mitogens
Sheep
Spleen
Thymus Gland
Dinitrofluorobenzene
Hemagglutinins
Interleukin-2
Interleukin-6
Mitogens