J Korean Orthop Assoc.  2001 Apr;36(2):127-134.

Gene Transfer Into Human Chondrocyte Derived Cells Using A Liposome Mediated Transfection System

• Han CW
• Kim WY
• Kim JY
• Ohm EY
• Kim JM

Abstract

PURPOSE: To introduce the CMV promoter driven luciferase and -galactosidase marker gene into previously permeabilized human chondrial cells.
MATERIALS AND METHODS
The cultured chondrocyte cells were transfected with a liposome/DNA mixture (pCMV-Luc or pSV40-lacZ). Cultured cells not transfected by liposome/DNA were used as a control. After forty-eight hours of incubation, the cells were used for reporter gene assays and the polymerase chain reaction (PCR).
RESULTS
Fifteen percent of the chondrocyte cells treated with liposome/ pSV40-lacZ DNA were positive for -gal staining. Chondrocyte cells transfected with pCMV-Luc yielded a 70-fold increase in luciferase activity over that of the control cells. A PCR product corresponding to the luciferase gene appeared only in the transfected chondrocyte cells. These results indicate that the human chondrocyte cells can be transfected with pCMV-Luc and pSV40-lacZ.
CONCLUSION
This system is particularly suitable for gene therapy, as well as for the use of genetically modified cartilage cells for resurfacing full thickness articular cartilage defects.

Keyword

Articular cartilage defect; Chondrocyte; Liposome; Transfection

MeSH Terms

Cartilage
Cartilage, Articular
Cells, Cultured
Chondrocytes*
DNA
Genes, Reporter
Genetic Therapy
Humans*
Liposomes*
Luciferases
Polymerase Chain Reaction
Transfection*
DNA
Liposomes
Luciferases