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Improved Transfection Efficiency and Metabolic Activity in Human Embryonic Stem Cell Using Non-Enzymatic Method

Kim CY, Hwang IK, Kang C, Chung EB, Jung CR, Oh H, Jeong YH, Moon SH, Kim JS, Hong KS, Park JH, Chung HM

Human embryonic stem cells (hESCs) are pluripotent cells widely used in conventional and regenerative medicine due to their ability to self-renew, proliferate and differentiate. Recently, genetic modification of stem cells...
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Epidermal Growth Factor (EGF)-Like Repeats and Discoidin I-Like Domains 3 (EDIL3): A Potential New Therapeutic Tool for the Treatment of Keloid Scars

Ryu YH, Lee YJ, Kim KJ, Lee SJ, Han YN, Rhie JW

In keloids, the mechanism underlying the excessive accumulation of extracellular matrix after injury of the skin is unclear, and there is no effective treatment because of the incomplete understanding of...
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Role of 15-hydroxyprostaglandin dehydrogenase down-regulation on the prognosis of hepatocellular carcinoma

Yang JE, Park E, Lee HJ, Kang HJ, Kim KM, Yu E, Lee D, Shim JH, Lim YS, Lee HC, Chung YH, Lee YS

BACKGROUND/AIMS: The role of prostaglandin E2 (PGE2) in the modulation of cell growth is well established in colorectal cancer. The aim of this study was to elucidate the significance of...
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Comparison of Various Transfection Methods in Human and Bovine Cultured Cells

Jin L, Kim D, Roh S

Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the...
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Sendai F/HN Viroplexes for Efficient Transfection of Leukemic T Cells

Kim JS, Lee YK, Jeong HY, Kang SJ, Kim MW, Ryu SH, Kim HS, Kim KS, Kim DE, Park YS

PURPOSE: Most chemical transfection reagents are ineffective for the transfection of cells in suspension, such as leukemic cell and stem cell lineages. We developed two different types of viroplexes, cationic...
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Modulation of osteoblastic/odontoblastic differentiation of adult mesenchymal stem cells through gene introduction: a brief review

Kim JY, Kim MR, Kim SJ

Bone tissue engineering is one of the important therapeutic approaches to the regeneration of bones in the entire field of regeneration medicine. Mesenchymal stem cells (MSCs) are actively discussed as...
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Comparison of Superparamagnetic Iron Oxide Labeling Efficiency between Poly-L-Lysine and Protamine Sulfate for Human Mesenchymal Stem Cells: Quantitative Analysis Using Multi-Echo T2* Magnetic Resonance Imaging

Suh JY, Lee JH, Lee CK, Shin JH, Choi CG, Kim JK

PURPOSE: To quantify in vitro labeling efficiency of protamine sulfate (PS) and poly-L-lysine (PLL) for labeling of human mesenchymal stem cells (hMSCs) with superparamagnetic iron oxide (SPIO) using multi-echo T2*...
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Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells

Kim W, Kim JH, Kong SY, Park MH, Sohn UD, Kim HJ

Neural stem cells (NSCs) have the ability to proliferate and differentiate into various types of cells that compose the nervous system. To study functions of genes in stem cell biology,...
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The Effect of Transfection of RET9 and RET51 on Apoptosis in SW48 and RKO Colon Cancer Cells

Seo HI, Park DI

BACKGROUND/AIMS: Dependent receptor can transmit both positive signal: proliferation, differentiation or migration; and negative signal: apoptosis. It depends on the presence of its ligand. This study was performed to determine...
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RET: A Multi-Faceted Gene in Human Cancer

Santoro M, Carlomagno F, Melillo RM

REarranged during Transfection (RET) gene encodes a receptor tyrosine kinase and it was initially discovered as an in vitro transforming gene. For many years, RET has been involved in papillary...
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Repeated Gene Transfection Impairs the Engraftment of Transplanted Porcine Neonatal Pancreatic Cells

Seo MK, Sun CL, Kim JW, Yoon KH, Lee SK

BACKGROUND: Previously, we reported that neonatal porcine pancreatic cells transfected with hepatocyte growth factor (HGF) gene in an Epstein-Barr virus (EBV)-based plasmid (pEBVHGF) showed improved proliferation and differentiation compared to...
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The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats

Ki KH, Park DY, Lee SH, Kim NY, Choi BM, Noh GJ

BACKGROUND: Small interfering RNAs (siRNAs) have been used to knockdown specific gene expression in various cells. Astrocytes and microglial cells play a key role in fundamental central nervous system functions...
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Membrane transducing activity of recombinant Hoxc8 protein and its possible application as a gene delivery vector

Kim ES, Kang M, Park HW, Kim MH

In order to examine whether the Hoxc8 protein can deliver nucleic acid into mammalian cells, we designed several Hoxc8-derived recombinant proteins to be synthesized as glutathione S-transferase (GST) fused forms...
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The Role of GADD45beta in Acute Myocardial Injury

Cho S, Hong JM, Lee JR, Lee HM, Oh BC, Lee JW

  • KMID: 1987452
  • Korean J Thorac Cardiovasc Surg.
  • 2008 Feb;41(1):25-33.
BACKGROUND: A critical shortage of donor organs has necessitated an investigation of new strategies to increase the availability of additional organs available for human transplantation. We investigated the amount of...
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Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells

Ahn HJ, Kim S, Nam HW

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar...
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Cotransplantation of Cord Blood Hematopoietic Stem Cells and Culture-Expanded and GM-CSF-/SCF-Transfected Mesenchymal Stem Cells in SCID Mice

Han JY, Goh RY, Seo SY, Hwang TH, Kwon HC, Kim SH, Kim JS, Kim HJ, Lee YH

Mesenchymal stem cells (MSC) are multipotent in nature and believed to facilitate the engraftment of hematopoietic stem cells (HSC) when transplanted simultaneously in animal studies and even in human trials....
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Adenoviral vector mediated in vivo gene transfer of BDNF promote functional recovery after facial nerve crush injury

Yang BE, Lee JH

  • KMID: 2328375
  • J Korean Assoc Oral Maxillofac Surg.
  • 2006 Aug;32(4):308-316.
Objectives Despite considerable advances in technique, experience and skill, the precise place of surgery in the treatment of facial nerve injury remains uncertain. We designed a facial nerve crush...
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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

Yang HW, Kim J, Yong TS, Park SJ

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase...
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Overexpression of SOX9 in mouse embryonic stem cells directs the immediate chondrogenic commitment

Kim JH, Do HJ, Yang HM, Oh JH, Choi SJ, Kim DK, Cha KY, Chung HM

  • KMID: 1093460
  • Exp Mol Med.
  • 2005 Aug;37(4):261-268.
Mouse embryonic stem (mES) cells are capable of undergoing chondrogenesis in vitro. To enhance this process, the human SOX9 (hSOX9) cDNA was delivered into mES cells and the clones overexpressing...
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Efficient gene delivery in differentiated human embryonic stem cells

Kim JH, Do HJ, Choi SJ, Cho HJ, Park KH, Yang HM, Lee SH, Kim DK, Kwack K, Oh SK, Moon SY, Cha KY, Chung HM

  • KMID: 1033099
  • Exp Mol Med.
  • 2005 Feb;37(1):36-44.
Human embryonic stem (hES) cells are capable of differentiating into pluralistic cell types, however, spontaneous differentiation generally gives rise to a limited number of specific differentiated cell types and a...
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