Korean J Anat.  2004 Dec;37(6):557-563.

The Effect of Iron Depletion on the Monocytic Adhesion, Differentiation and SR-A Expression

• Seo GS
• Choi SC
• Choi EY
• Kim TH
• Nah YH
• Oh JM
• Kim SW
• Jun CD

Abstract

Maintenance of cellular iron homeostasis is a prerequisite for proliferation and differentiation of cells, and is also a central role in the regulation of immune function. Monocyte-macrophages play an important roles in host defense, particularly in the inflammatory process of acute and chronic disease. The reason that an iron is important in these cell is because an iron is indispensable in a generation of hydroxyl radical for bacterium killing. Because of the role of iron in the monocytic THP-1 cell differentiation is not become clear, we investigated whether THP-1 cell can differentiate to macrophage-like cell using of iron and iron chelator which cause iron depletion. The cell differentiation was not able to observe by iron treatment, by the way, the cell adhesion was increased in DFO treated monocyte and cellular pseodopodial extension, change of a nucleus-cytoplasmic ratio were showed in Differential interference contrast (DIC) and Giemsa staining, and it was inhibited by ferric citrate (FC). Increased polystyrene bead phagocytosis by DFO treatment of THP-1 cell were detected through FACS and rhodamine-phallodin staining. The SR-A expression, which was a cell differentiation marker, was increased by DFO treatment of THP-1 cell. These results suggest that iron depletion by DFO can promote THP-1 cell diffentiation into macrophage-like cell, and this may carrying out important role in the immune response.

Keyword

Iron deficiency; Host immune response; Desferrioxamine; Monocyte; Macrophage; Cell differentiation

MeSH Terms

Azure Stains
Cell Adhesion
Cell Differentiation
Chronic Disease
Citric Acid
Deferoxamine
Homeostasis
Homicide
Hydroxyl Radical
Iron*
Macrophages
Monocytes
Phagocytosis
Polystyrenes
Azure Stains
Citric Acid
Deferoxamine
Hydroxyl Radical
Iron
Polystyrenes