Korean J Anat.
2004 Dec;37(6):539-547.
Analysis of Genomic Imbalances in Korean Gastric Carcinoma Using Comparative Genomic Hybridization
- Affiliations
-
- 1Department of Anatomy, College of Medicine, Ewha Womans University, Korea.
- 2Institute of Human Genetics, Department of Anatomy, College of Medicine, Korea University, Seoul, Korea. parksh@korea.ac.kr
Abstract
- Chromosomal abnormalities, which are valuable markers for diagnosis and prognosis of cancer, provide useful clues in characterizing cancer at molecular level. Gastric cancer is the major cause of cancer deaths in Asian countries, including Korea. Genetic changes during the progression and metastasis of gastric cancer remain unclear. Recently, technique of degenerate oligonucleotide primed (DOP) PCR-comparative genomic hybridization (CGH) permits genetic imbalances screening of the entire genome using only small amounts of tumor DNA. In non-metastatic gastric cancers the common sites of copy number increases were detected at 8q (64%), 4p12-q24 (64%), 5p13-q23 (64%), 13q21-q32 (64%), 6q11-q21 (55%), 7q(50%), 14q11.2-q21 (45%), 3q11-q13.3 (41%), and 2q23-q32 (41%). In metastatic gastric cancers, the frequent sites of gains were detected at 8p21-qter (60%), 5 (54%), 20 (42%), 6pter-q24 (51%), 1q21-qter (46%), 3p14-qter (46%), 22q (46%), and 4 (43%). Deletion or chromosomal loss was found to be less frequent in this study. The frequent sites of copy number decreases were detected at 1p34-pter (23%), 16q23-q24 (18%), and 19q13 (18%) in non-metastatic gastric cancers. In metastatic gastric cancers, chromosome losses were detected at X (37%), 1p33-pter (37%), and 16p (23%). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes respectively involved in the tumorigenesis of gastric cancer.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Chromosomal gains and losses in primary ovarian carcinomas by comparative genomic hybridization
- A Case of Identification of Marker Chromosome by comparative genomic hybridization and fluorescence in situ hybridization
- Current Status and Future Clinical Applications of Array.based Comparative Genomic Hybridization
- Analysis of Chromosomal Aberrations in Lung Cancer Cell Line, NCI-H1373
- Array-based Comparative Genomic Hybridization and Its Application to Cancer Genomes and Human Genetics
