Korean J Obstet Gynecol.
2003 Jan;46(1):38-43.
Chromosomal gains and losses in primary ovarian carcinomas by comparative genomic hybridization
- Affiliations
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- 1Department of Obstetrics and Gynecology, College of Medicine, Korea University, Korea.
- 2Sewha Pediatric Clinic, Seoul, Korea.
Abstract
OBJECTIVE
Comparative genomic hybridization was performed to evaluate DNA sequence copy number changes in human ovarian carcinomas from paraffin-embedded tissue blocks.
PATIENTS AND METHODS
DNA from 20 cases of primary ovarian carcinomas underwent comparative genomic hybridization to evaluate the extent of genetic gains or losses in a test sample.
RESULTS
In thirteen cases of 20 samples, varying degree of genetic imbalances was observed. Of the remaining 7 cases, two revealed normal, five failed to yield a result. Most common genetic imbalances are 8q22.2-q24 site amplification and 12p site amplification, where c-myc gene and k-ras gene respectively are included. Second most common site of genetic imbalance is 7p21-pter site deletion.
CONCLUSION
Our results have shown many chromosomal alterations in human ovarian carcinomas, and these sites are known previously as oncogene or tumor-suppression gene, and some sites are not known specific cancer associated sites. Our data can be useful for screening chromosomal changes and molecular mechanism of human ovarian carcinogenesis.
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